anti-Human BIM antibody for Western Blotting

Recommended BIM Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • LOC100345483
  • BCL2L11
  • Bcl2l11
Reactivity
Human, Mouse (Murine), Rat (Rattus)
333
235
207
9
6
6
4
3
3
1
1
Host
Rabbit
284
29
16
9
7
Clonality
Polyclonal
Conjugate
This BIM antibody is un-conjugated
16
14
11
9
9
9
5
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
279
139
115
80
72
47
33
28
17
7
5
4
3
2
2
2
2
Options
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Catalog No. ABIN222941
$ 245.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.066084 ABIN967649 WB Rabbit Ig Fraction AA 22-40 Log in to see Polyclonal 6
3.066084 ABIN652493 FACS IHC (p) WB Rabbit Ig AA 134-160, Center Log in to see Polyclonal 2
3.066084 ABIN2707367 IHC WB Rabbit N-Term Log in to see Polyclonal
3.066084 ABIN388101 IHC (p) WB Rabbit Ig AA 130-165, BH3 Domain Log in to see Polyclonal 6
3.066084 ABIN453531 EIA WB Rabbit Middle Region Log in to see Polyclonal 2
3.066084 ABIN1533217 IHC ELISA WB Rabbit IgG AA 1-50 Log in to see Polyclonal
3.066084 ABIN250248 ELISA WB Goat C-Term Log in to see Polyclonal 2
3.066084 ABIN361752 ICC IF WB Rabbit Log in to see Polyclonal 2
3.066084 ABIN4284640 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 7
3.066084 ABIN2485927 IHC WB Atto 390 Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485935 IHC WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485940 IHC WB PE-Atto 594 Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485928 IHC WB Atto 488 Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485936 IHC WB APC Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485934 IHC WB Atto 700 Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485942 IHC WB PE Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485929 IHC WB Atto 565 Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485930 IHC WB Atto 594 Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485931 IHC WB Atto 633 Rabbit Log in to see Polyclonal 2
3.066084 ABIN2485932 IHC WB Atto 655 Rabbit Log in to see Polyclonal 2

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(333), (235), (207), (9), (6), (6), (4), (3), (3), (1), (1)
Host Rabbit
(284), (29), (16), (9), (7)
Clonality
Polyclonal
Conjugate This BIM antibody is un-conjugated
(16), (14), (11), (9), (9), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(279), (139), (115), (80), (72), (47), (33), (28), (17), (7), (5), (4), (3), (2), (2), (2), (2)
Pubmed 3 references available
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Product Details anti-BIM Antibody

Target Details BIM Application Details Handling References for anti-BIM antibody (ABIN222941) Images
Blocking Peptide Blocking peptide for this product available: ABIN412620
Specificity The antibody detects a 20 kDa Bim/BOD in samples from human, mouse and rat origins.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 25 of human Bim
Isotype IgG

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling References for anti-BIM antibody (ABIN222941) Images back to top
Antigen
Alternative Name Bim/Bod (BCL2L11 Antibody Abstract)
Background Members of the Bcl-2 family proteins all contain Bcl-2 homology domains including BH1, BH2, BH3, and BH4. The BH3 domain is known to be important for the cell killing activity of Bcl-2 members including Bad, Bax, Bid, Bik, and the recently identified BH3 domain containing protein designated Bim/BOD. Bim/BOD interacts with diverse members of the Bcl-2 sub-family including Bcl-2, Bcl-x, and Bcl-w, to induce apoptosis. There are three splice variants: BimL, BimS, and BimEL, all of which share homology in their C-terminal BH3 domains. Of the isoforms, BimS is the most potent inducer of apoptosis. The mRNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Synonyms: Bcl-2-like protein 11, BCL2L11
Gene ID 10018
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling References for anti-BIM antibody (ABIN222941) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL). However, the optimal conditions should be determined individually. The antibody detects a 20 kDa Bim/BOD in samples from human, mouse and rat origins. Jurkat cell lysate can be used as a positive control.
Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details References for anti-BIM antibody (ABIN222941) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-Bim/BOD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BIM antibody (ABIN222941)

Product Details anti-BIM Antibody Target Details BIM Application Details Handling Images back to top
Product cited in:

Loh, Liou, Jiau, Cheng, Shih, Chen: "Hepatic effects among workers exposed to ethylene glycol monoethyl ether acetate." in: Industrial health, Vol. 46, Issue 5, pp. 463-9, 2008

Chen, Huang, Wang: "Deficiency of Bim in dendritic cells contributes to overactivation of lymphocytes and autoimmunity." in: Blood, Vol. 109, Issue 10, pp. 4360-7, 2007

Liou, Zhou, Pei, Lim, Yin, Chen: "BimEL up-regulation potentiates AIF translocation and cell death in response to MPTP." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 10, pp. 1350-2, 2005

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling References for anti-BIM antibody (ABIN222941) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (ABIN222941) Western blot analysis of Bim expression in K526 cell lysate.