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anti-Mouse (Murine) BIM antibody for Immunohistochemistry (Frozen Sections)

Recommended BIM Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • 1500006F24Rik
  • BAM
  • Bim
  • bim
  • BIM
  • BIMEL
  • BimL
  • Bod
  • BOD
Reactivity
Human, Mouse (Murine), Rat (Rattus)
308
169
138
4
2
2
2
2
2
Host
Rabbit
264
30
16
13
7
Clonality
Polyclonal
Conjugate
This BIM antibody is un-conjugated
16
14
10
8
8
8
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
255
135
115
64
51
38
36
26
14
8
5
3
3
2
2
2
Supplier
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Catalog No. ABIN264853
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008880001 ABIN264852 IHC (fro) WB Rabbit IgG Log in to see Polyclonal 2
0.0008880001 ABIN187549 FACS IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see 14A8

Top referenced anti-BIM antibody for Immunohistochemistry (Frozen Sections)

Similar anti-BIM Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 247 Antibodies 119 Antibodies 102 Antibodies
Immunostaining (ISt) 2 Antibodies
Immunoprecipitation (IP) 9 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies 26 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 129 Antibodies 65 Antibodies 57 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies 26 Antibodies 26 Antibodies
Immunofluorescence (IF) 45 Antibodies 40 Antibodies 31 Antibodies

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(308), (169), (138), (4), (2), (2), (2), (2), (2)
Host Rabbit
(264), (30), (16), (13), (7)
Clonality
Polyclonal
Conjugate This BIM antibody is un-conjugated
(16), (14), (10), (8), (8), (8), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(255), (135), (115), (64), (51), (38), (36), (26), (14), (8), (5), (3), (3), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-BIM Antibody

Target Details BIM Application Details Handling References for anti-BIM Antibody (ABIN264853) Images
Specificity Detects a 23 kDa protein corresponding to the molecular mass of Bim on SDS PAGE immunoblots.
Purification Affinity Chromatography
Immunogen Internal central amino acids of Human Bim
Isotype IgG

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling References for anti-BIM Antibody (ABIN264853) Images back to top
Antigen
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS
Gene ID 10018
NCBI Accession NP_001191035
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling References for anti-BIM Antibody (ABIN264853) Images back to top
Application Notes Western blot: 1: 1000. Immunohistochemistry on frozen sections: 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details References for anti-BIM Antibody (ABIN264853) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BIM Antibody (ABIN264853)

Product Details anti-BIM Antibody Target Details BIM Application Details Handling Images back to top
Background publications

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998

OConnor, Strasser, OReilly, Hausmann, Adams, Cory, Huang: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling References for anti-BIM Antibody (ABIN264853) back to top
Supplier Images
 image for anti-BIM antibody (BCL2-Like 11 (Apoptosis Facilitator)) (ABIN264853) anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody
 image for anti-BIM antibody (BCL2-Like 11 (Apoptosis Facilitator)) (ABIN264853) anti-BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody (Image 2)