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anti-Mouse (Murine) Cyclin A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Cyclin A Antibody (supplied by: Log in to see )

Antigen
Cyclin A2 (CCNA2) Antibodies
  • AA408589
  • cb671
  • Ccn-1
  • ccn1
  • Ccn1
  • CCN1
  • ccna
  • Ccna
  • CCNA
  • ccna1
  • ccna2
  • chunp6924
  • Cyca
  • CYCA
  • CycA2
  • Cyclin-A
  • Cyclin-A2
  • cyclinA
  • wu:fi36f03
Epitope
C-Term
28
15
5
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
133
48
33
31
5
3
3
2
2
2
2
2
1
Host
Rabbit
98
59
1
Clonality
Polyclonal
Conjugate
This Cyclin A antibody is un-conjugated
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
128
52
45
42
31
28
19
12
5
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN181287
$ 554.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089715846 ABIN265377 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.00089715846 ABIN670281 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.00089715846 ABIN2855544 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089715846 ABIN121516 EIA IHC (p) WB Rabbit Log in to see Polyclonal 3
0.00089715846 ABIN2855543 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.00089715846 ABIN184530 IHC (fro) IHC (p) IP WB Mouse IgG2a Log in to see E67-1
0.00089715846 ABIN1733430 IF IHC (p) IP ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Cyclin A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Cyclin A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 11 Antibodies 12 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 11 Antibodies
Immunofluorescence (IF) 14 Antibodies 19 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 8 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 33 Antibodies
Flow Cytometry (FACS) 2 Antibodies

General

Antigen Cyclin A2 (CCNA2) Antibodies
Epitope C-Term
(28), (15), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(133), (48), (33), (31), (5), (3), (3), (2), (2), (2), (2), (2), (1)
Host Rabbit
(98), (59), (1)
Clonality
Polyclonal
Conjugate This Cyclin A antibody is un-conjugated
(2), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(128), (52), (45), (42), (31), (28), (19), (12), (5), (4), (4), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Cyclin A Antibody

Target Details Cyclin A Application Details Handling References for anti-Cyclin A Antibody (ABIN181287) Images
Specificity Highly specific for Cyclin A.
Cellular Localization: Nuclear. Mol. Wt. of Antigen: 60 kDa.
Purification Protein A affinity purified immunoglobulin fraction
Immunogen A synthetic peptide from the C-terminus of human cyclin A.

Target Details Cyclin A

Product Details anti-Cyclin A Antibody Application Details Handling References for anti-Cyclin A Antibody (ABIN181287) Images back to top
Antigen
Alternative Name Cyclin A2 (CCNA2 Antibody Abstract)
Background Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expre ssion of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G 2-phase. The D and E type cyclins regulate the passage of G 1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G 2-phase.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2
Molecular Weight 60kD.
Gene ID 890
NCBI Accession NP_001228
UniProt P20248
Research Area Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling

Application Details

Product Details anti-Cyclin A Antibody Target Details Cyclin A Handling References for anti-Cyclin A Antibody (ABIN181287) Images back to top
Application Notes Western Blotting (5-10 μg/mL for 2hrs at RT)Immunoprecipitation (Denatured, Use ProteinA: 10 g/mg protein lysate)Immunohistology (Formalin/paraffin 5-10 μg/mL for 30 min at RT. Staining of formalin-fixedtissues REQUIRES boilingtissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for20 min. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details References for anti-Cyclin A Antibody (ABIN181287) Images back to top
Concentration 1 mg/mL
Buffer 10 mM PBS pH 7.4 with 0.09 % sodium azide as preservative and 0.2 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid Repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C for up to one month or in aliquots at -20 °C for longer.

References for anti-Cyclin A Antibody (ABIN181287)

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling Images back to top
Background publications

Dutta, Chandra, Leiter, Lester: "Cyclins as markers of tumor proliferation: immunocytochemical studies in breast cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5386-90, 1995

Gong, Bhatia, Traganos, Darzynkiewicz: "Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry." in: Leukemia, Vol. 9, Issue 5, pp. 893-9, 1995

Images

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling References for anti-Cyclin A Antibody (ABIN181287) back to top
Supplier Images
 image for anti-Cyclin A antibody (Cyclin A2) (C-Term) (ABIN181287) anti-Cyclin A2 (CCNA2) (C-Term) antibody