You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Non Human Primates GRIA1 antibody for Immunofluorescence

Recommended GRIA1 Antibody (supplied by: Log in to see )

Antigen
Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) Antibodies
  • 2900051M01Rik
  • Glr-1
  • Glr1
  • GluA1
  • GLUH1
  • Glur-1
  • gluR-A
  • GluR-A
  • gluR-K1
  • glur1
  • Glur1
  • GLUR1
  • GLURA
  • GluRA
  • GRIA1
  • HBGR1
  • HIPA1
Epitope
pSer845
45
36
28
26
22
21
15
14
13
13
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Non Human Primates, Rat (Rattus)
199
198
162
8
7
6
3
2
1
Host
Rabbit
214
50
5
Clonality
Polyclonal
Conjugate
This GRIA1 antibody is un-conjugated
7
6
5
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
201
82
51
45
41
40
26
9
5
3
3
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN372653
$ 412.50
Plus shipping costs $45.00

Top referenced anti-GRIA1 antibody for Immunofluorescence

Similar anti-GRIA1 Antibodies

Application / Reactivity Mouse (Murine) Rat (Rattus) Human Non Human Primates
Western Blotting (WB) 111 Antibodies 147 Antibodies 137 Antibodies 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies 20 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 69 Antibodies 44 Antibodies 49 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies 4 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies 45 Antibodies

General

Antigen Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) Antibodies
Epitope pSer845
(45), (36), (28), (26), (22), (21), (15), (14), (13), (13), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Non Human Primates, Rat (Rattus)
(199), (198), (162), (8), (7), (6), (3), (2), (1)
Host Rabbit
(214), (50), (5)
Clonality
Polyclonal
Conjugate This GRIA1 antibody is un-conjugated
(7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(201), (82), (51), (45), (41), (40), (26), (9), (5), (3), (3), (1), (1), (1)
Pubmed 15 references available
Supplier Log in to see

Product Details anti-GRIA1 Antibody

Target Details GRIA1 Application Details Handling References for anti-GRIA1 Antibody (ABIN372653) Images
Specificity Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is s completely eliminated by treatment with lambda-Ptase.
Cross-Reactivity (Details) Species reactivity (expected):Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.
Isotype IgG

Target Details GRIA1

Product Details anti-GRIA1 Antibody Application Details Handling References for anti-GRIA1 Antibody (ABIN372653) Images back to top
Antigen
Alternative Name Glutamate Receptor 1 / GLUR1 (GRIA1 Antibody Abstract)
Background The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990, Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993, Mammen et al., 1999, Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory (Soderling and Derkach, 2000).Synonyms: AMPA-selective glutamate receptor 1, AMPA1, GLUH1, GRIA1, GluR-1, GluR-A, GluR-K1, Glutamate receptor ionotropic
Gene ID 50592
NCBI Accession NP_113796
UniProt P19490
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-GRIA1 Antibody Target Details GRIA1 Handling References for anti-GRIA1 Antibody (ABIN372653) Images back to top
Application Notes Western blot: 1/1000. Immunofluorescence. Immunohistochemitsry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GRIA1 Antibody Target Details GRIA1 Application Details References for anti-GRIA1 Antibody (ABIN372653) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C/-80 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C or (in aliquots) at -80 °C for long term.

References for anti-GRIA1 Antibody (ABIN372653)

Product Details anti-GRIA1 Antibody Target Details GRIA1 Application Details Handling Images back to top
Product cited in:

Shen, Fu, Cheng, Fu, Ip: "Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 464-72, 2013

Santini, Feyder, Gangarossa, Bateup, Greengard, Fisone et al.: "Dopamine- and cAMP-regulated phosphoprotein of 32-kDa (DARPP-32)-dependent activation of extracellular signal-regulated kinase (ERK) and mammalian target of rapamycin complex 1 (mTORC1) signaling in ..." in: The Journal of biological chemistry, Vol. 287, Issue 33, pp. 27806-12, 2012

Sears, Liu, Narayanan, Sharf, Yeckel, Laubach, Aghajanian, DiLeone: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, 2010

Rebholz, Nishi, Liebscher, Nairn, Flajolet, Greengard: "CK2 negatively regulates Galphas signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 14096-101, 2009

Santini, Heiman, Greengard, Valjent, Fisone: "Inhibition of mTOR signaling in Parkinson's disease prevents L-DOPA-induced dyskinesia." in: Science signaling, Vol. 2, Issue 80, pp. ra36, 2009

Zheng, Keifer: "PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning." in: Journal of neurophysiology, Vol. 101, Issue 5, pp. 2539-49, 2009

Meyer, Richer, Benkovic, Hayashi, Kansy, Hale, Moy, Kim, OCallaghan, Tsai, Greengard, Nairn, Cowan, Miller, Antich, Bibb: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, 2008

Nishi, Kuroiwa, Miller, OCallaghan, Bateup, Shuto, Sotogaku, Fukuda, Heintz, Greengard, Snyder: "Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 42, pp. 10460-71, 2008

Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, 2008

Background publications

Soderling, Derkach: "Postsynaptic protein phosphorylation and LTP." in: Trends in neurosciences, Vol. 23, Issue 2, pp. 75-80, 2000

Mammen, Kameyama, Roche, Huganir: "Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin-dependent kinase II." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32528-33, 1998

Roche, OBrien, Mammen, Bernhardt, Huganir: "Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit." in: Neuron, Vol. 16, Issue 6, pp. 1179-88, 1996

Hollmann, Heinemann: "Cloned glutamate receptors." in: Annual review of neuroscience, Vol. 17, pp. 31-108, 1994

McGlade-McCulloh, Yamamoto, Tan, Brickey, Soderling: "Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II." in: Nature, Vol. 362, Issue 6421, pp. 640-2, 1993

Keinänen, Wisden, Sommer, Werner, Herb, Verdoorn, Sakmann, Seeburg: "A family of AMPA-selective glutamate receptors." in: Science (New York, N.Y.), Vol. 249, Issue 4968, pp. 556-60, 1990

Images

Product Details anti-GRIA1 Antibody Target Details GRIA1 Application Details Handling References for anti-GRIA1 Antibody (ABIN372653) back to top
Supplier Images
 image for anti-GRIA1 antibody (Glutamate Receptor, Ionotropic, AMPA 1) (pSer845) (ABIN372653) anti-Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) (pSer845) antibody