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anti-Human MAPKSP1 antibody for Western Blotting

Recommended MAPKSP1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) Antibodies
  • AW556229
  • MAP2K1IP1
  • Map2k1ip1
  • MAPBP
  • Mapbp
  • mapksp1
  • Mapksp1
  • MAPKSP1
  • MP1
  • Mp1
  • PRO0633
  • Ragulator3
  • zgc:110207
Epitope
AA 1-88
19
18
8
2
2
2
2
Reactivity
Human
58
39
21
Host
Mouse
53
5
Clonality
Monoclonal
Conjugate
This MAPKSP1 antibody is un-conjugated
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
43
24
21
18
17
14
11
2
1
1
1
Supplier
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Catalog No. ABIN393554
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN189551 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y130
0.13476625 ABIN2788404 WB Rabbit N-Term Log in to see Polyclonal
0.13476625 ABIN1859761 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-124 Log in to see Polyclonal
0.13476625 ABIN522095 ELISA WB Mouse IgG1 kappa AA 1-87, partial Log in to see 2A4
0.13476625 ABIN2489507 IHC (fp) WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN3016323 IHC WB Rabbit Log in to see Polyclonal
0.0011760078 ABIN1833654 IHC (p) WB Rabbit IgG AA 1-124 Log in to see Polyclonal
0.0011760078 ABIN1494754 WB Rabbit N-Term Log in to see Polyclonal
0.0011760078 ABIN563729 ELISA WB Mouse AA 1-87, partial Log in to see Polyclonal
0.0011760078 ABIN204412 ELISA WB Mouse IgG2b AA 1-124 Log in to see
0.0011760078 ABIN2880364 WB Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN2624768 ELISA WB Biotin Rabbit IgG AA 1-124 Log in to see Polyclonal
0.0011760078 ABIN2624769 WB FITC Rabbit IgG AA 1-124 Log in to see Polyclonal
0.0011760078 ABIN1088378 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN1088377 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN1700505 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN1711522 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN1714007 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN2307529 ELISA WB Mouse IgG1 kappa AA 1-88 Log in to see 2A4
0.0011760078 ABIN2320776 IC IHC ELISA WB Rabbit IgG AA 1-124 Log in to see Polyclonal

Similar anti-MAPKSP1 Antibodies

Application / Reactivity Human
ELISA 14 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 45 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) Antibodies
Epitope AA 1-88
(19), (18), (8), (2), (2), (2), (2)
Reactivity Human
(58), (39), (21)
Host Mouse
(53), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPKSP1 antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(43), (24), (21), (18), (17), (14), (11), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-MAPKSP1 Antibody

Target Details MAPKSP1 Application Details Handling References for anti-MAPKSP1 Antibody (ABIN393554) Images
Specificity MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2A4
Isotype IgG1 kappa

Target Details MAPKSP1

Product Details anti-MAPKSP1 Antibody Application Details Handling References for anti-MAPKSP1 Antibody (ABIN393554) Images back to top
Antigen
Alternative Name MAP2K1IP1 (MAPKSP1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 13623 DA
Gene ID 12406
NCBI Accession NP_068805
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Handling References for anti-MAPKSP1 Antibody (ABIN393554) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein.

Restrictions For Research Use only

Handling

Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details References for anti-MAPKSP1 Antibody (ABIN393554) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-MAPKSP1 Antibody (ABIN393554)

Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details Handling Images back to top
Background publications

Sancak, Bar-Peled, Zoncu, Markhard, Nada, Sabatini: "Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids." in: Cell, Vol. 141, Issue 2, pp. 290-303, 2010

Vomastek, Iwanicki, Schaeffer, Tarcsafalvi, Parsons, Weber: "RACK1 targets the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to link integrin engagement with focal adhesion disassembly and cell motility." in: Molecular and cellular biology, Vol. 27, Issue 23, pp. 8296-305, 2007

Brahma, Dalby: "Regulation of protein phosphorylation within the MKK1-ERK2 complex by MP1 and the MP1*P14 heterodimer." in: Archives of biochemistry and biophysics, Vol. 460, Issue 1, pp. 85-91, 2007

Teis, Taub, Kurzbauer, Hilber, de Araujo, Erlacher, Offterdinger, Villunger, Geley, Bohn, Klein, Hess, Huber: "p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis." in: The Journal of cell biology, Vol. 175, Issue 6, pp. 861-8, 2006

Villegas, Njaine, Linden, Carri: "Glial-derived neurotrophic factor (GDNF) prevents ethanol (EtOH) induced B92 glial cell death by both PI3K/AKT and MEK/ERK signaling pathways." in: Brain research bulletin, Vol. 71, Issue 1-3, pp. 116-26, 2006

Images

Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details Handling References for anti-MAPKSP1 Antibody (ABIN393554) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPKSP1 antibody (Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1) (AA 1-88) (ABIN393554) Western Blot analysis of MAP2K1IP1 expression in transfected 293T cell line by MAP2K1...