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|Application / Reactivity||Human|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||9 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||33 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) Antibodies|
|Epitope||AA 1-88 Alternatives|
|Conjugate||This MAPKSP1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAPKSP1 AntibodyTarget Details MAPKSP1 Application Details Handling References for anti-MAPKSP1 Antibody (ABIN393554) Images
|Specificity||MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Purification||This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
Target Details MAPKSP1Product Details anti-MAPKSP1 Antibody Application Details Handling References for anti-MAPKSP1 Antibody (ABIN393554) Images back to top
|Alternative Name||MAP2K1IP1 (MAPKSP1 Antibody Abstract)|
|Background||Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1|
|Molecular Weight||13623 DA|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure|
Application DetailsProduct Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Handling References for anti-MAPKSP1 Antibody (ABIN393554) Images back to top
|Application Notes||Western blot = 1:500-1000|
Background: This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details References for anti-MAPKSP1 Antibody (ABIN393554) Images back to top
|Buffer||PBS with 0.09% (w/v) sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles|
|Expiry Date||6 months|
References for anti-MAPKSP1 Antibody (ABIN393554)Product Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details Handling Images back to top
Sancak, Bar-Peled, Zoncu et al.: "Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids." in: Cell, Vol. 141, Issue 2, pp. 290-303, 2010 (PubMed).
Vomastek, Iwanicki, Schaeffer et al.: "RACK1 targets the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to link integrin engagement with focal adhesion disassembly and cell motility." in: Molecular and cellular biology, Vol. 27, Issue 23, pp. 8296-305, 2007 (PubMed).
Brahma, Dalby: "Regulation of protein phosphorylation within the MKK1-ERK2 complex by MP1 and the MP1*P14 heterodimer." in: Archives of biochemistry and biophysics, Vol. 460, Issue 1, pp. 85-91, 2007 (PubMed).
Teis, Taub, Kurzbauer et al.: "p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis." in: The Journal of cell biology, Vol. 175, Issue 6, pp. 861-8, 2006 (PubMed).
Villegas, Njaine, Linden et al.: "Glial-derived neurotrophic factor (GDNF) prevents ethanol (EtOH) induced B92 glial cell death by both PI3K/AKT and MEK/ERK signaling pathways." in: Brain research bulletin, Vol. 71, Issue 1-3, pp. 116-26, 2006 (PubMed).
ImagesProduct Details anti-MAPKSP1 Antibody Target Details MAPKSP1 Application Details Handling References for anti-MAPKSP1 Antibody (ABIN393554) back to top