anti-Human MTOR antibody for Dot Blot

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
pThr2446
66
36
27
23
21
15
15
13
12
10
8
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
378
201
198
18
9
8
7
7
7
7
4
2
2
2
1
1
Host
Rabbit
324
49
4
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
8
7
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Dot Blot (DB)
253
114
99
75
57
52
37
22
8
7
7
7
3
2
2
2
1
1
1
Options
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Catalog No. ABIN1881353
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2794213 DB Rabbit Ig Fraction pThr2446 Log in to see Polyclonal

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope pThr2446
(66), (36), (27), (23), (21), (15), (15), (13), (12), (10), (8), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(378), (201), (198), (18), (9), (8), (7), (7), (7), (7), (4), (2), (2), (2), (1), (1)
Host Rabbit
(324), (49), (4), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This MTOR antibody is un-conjugated
(8), (8), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Dot Blot (DB)
(253), (114), (99), (75), (57), (52), (37), (22), (8), (7), (7), (7), (3), (2), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN1881353) Images
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Immunogen This FRAP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2446 of human FRAP1.
Clone RB13089

Target Details MTOR

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Antigen
Alternative Name FRAP1 (FRAP1 Antibody Abstract)
Background FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.

Synonyms: MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR antibody (ABIN1881353) Images back to top
Application Notes DB: 1:500
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR antibody (ABIN1881353) Images back to top
Format Liquid
Concentration 0.45 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

References for anti-MTOR antibody (ABIN1881353)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Pillai, Sundaresan, Kim, Gupta, Rajamohan, Pillai, Samant, Ravindra, Isbatan, Gupta: "Exogenous NAD blocks cardiac hypertrophic response via activation of the SIRT3-LKB1-AMP-activated kinase pathway." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3133-44, 2010

Dowling, Zakikhani, Fantus, Pollak, Sonenberg: "Metformin inhibits mammalian target of rapamycin-dependent translation initiation in breast cancer cells." in: Cancer research, Vol. 67, Issue 22, pp. 10804-12, 2007

Bai, Ma, Liu, Shen, Wang, Liu, Jiang: "Rheb activates mTOR by antagonizing its endogenous inhibitor, FKBP38." in: Science (New York, N.Y.), Vol. 318, Issue 5852, pp. 977-80, 2007

Zhou, Wulfkuhle, Zhang, Gu, Yang, Deng, Margolick, Liotta, Petricoin, Zhang: "Activation of the PTEN/mTOR/STAT3 pathway in breast cancer stem-like cells is required for viability and maintenance." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 41, pp. 16158-63, 2007

Images

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Supplier Images
Dot Blot (DB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pThr2446) antibody (ABIN1881353) Dot blot analysis of anti-FRAP1-pT2446 Phospho-specific Pab (Cat.AP3433a) on nitrocel...