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anti-Mouse (Murine) MTOR antibody for Immunofluorescence

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • 2610315D21Rik
  • AI327068
  • flat
  • FRAP
  • frap1
  • Frap1
  • FRAP1
  • FRAP2
  • RAFT1
  • RAPT1
  • tor
  • wu:fc22h08
Epitope
N-Term
66
24
24
23
22
21
14
13
13
9
7
6
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
340
164
151
8
2
2
2
1
1
Host
Rabbit
292
47
2
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
221
112
92
63
51
44
32
20
8
7
6
3
2
2
2
2
2
1
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Catalog No. ABIN501014
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3121241 ABIN968383 IF WB Mouse IgG1 AA 185-290 Log in to see 30-RAFT1 5
0.3121241 ABIN1531447 IF IHC ELISA Rabbit IgG pSer2481 Log in to see Polyclonal
0.3121241 ABIN2705013 IC IF WB Rabbit C-Term Log in to see Polyclonal
0.3121241 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010962716 ABIN196962 IF IHC (p) WB Rabbit pSer2448 Log in to see Polyclonal 4
0.0010962716 ABIN968384 IF WB Mouse IgG1 AA 185-290 Log in to see 30-RAFT1 5
0.0010962716 ABIN319329 IF IHC (p) WB Rabbit Ser2448 Log in to see Polyclonal 3
0.0010962716 ABIN303334 EIA IF IHC (p) WB Rabbit AA 2440-2457 Log in to see Polyclonal
0.0010962716 ABIN362868 IF IHC WB Rabbit IgG AA 2446-2450 Log in to see Polyclonal 1
0.0010962716 ABIN362262 IF IHC Rabbit IgG pSer2448 Log in to see Polyclonal 3
0.0010962716 ABIN498610 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010962716 ABIN302811 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010962716 ABIN1876705 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010962716 ABIN2470319 IF IHC ELISA WB Rabbit AA 1189-1199 Log in to see Polyclonal
0.0010962716 ABIN1737123 IF IHC (p) ELISA WB Rabbit IgG AA 2440-2457 Log in to see Polyclonal
0.0010962716 ABIN3183004 ELISA IF IHC Rabbit IgG pSer2481 Log in to see Polyclonal
0.0010962716 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Log in to see Polyclonal
0.0010962716 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Log in to see Polyclonal
0.0010962716 ABIN2755575 IC IF IP IHC WB Rabbit C-Term, pSer2448 Log in to see Polyclonal
0.0010962716 ABIN1842805 ICC IF IHC WB Rabbit pSer2481 Log in to see Polyclonal

Top referenced anti-MTOR antibody for Immunofluorescence

Similar anti-MTOR Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
ELISA 33 Antibodies 93 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 8 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 85 Antibodies
Dot Blot (DB) 2 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope N-Term
(66), (24), (24), (23), (22), (21), (14), (13), (13), (9), (7), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(340), (164), (151), (8), (2), (2), (2), (1), (1)
Host Rabbit
(292), (47), (2)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(221), (112), (92), (63), (51), (44), (32), (20), (8), (7), (6), (3), (2), (2), (2), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN501014) Images
Specificity This antibody detects mTOR / FRAP1.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Immunogen TOR antibody was raised against a 15 amino acid peptide from near the amino terminus of human TOR.
Isotype IgG

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR Antibody (ABIN501014) Images back to top
Antigen
Alternative Name mTOR / FRAP1 (FRAP1 Antibody Abstract)
Background The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin (2,3). Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 4). TOR is normally associated with the regulatory proteins RAPTOR and GbetaL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis (4).Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR Antibody (ABIN501014) Images back to top
Application Notes ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR Antibody (ABIN501014) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-MTOR Antibody (ABIN501014)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Background publications

Fingar, Blenis: "Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression." in: Oncogene, Vol. 23, Issue 18, pp. 3151-71, 2004

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003

Cardenas, Zhu, Heitman: "Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin." in: Current opinion in nephrology and hypertension, Vol. 4, Issue 6, pp. 472-7, 1996

Sabatini, Erdjument-Bromage, Lui, Tempst, Snyder: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN501014) back to top
Supplier Images
Immunofluorescence (IF) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (N-Term) (ABIN501014) Immunocytochemistry of TOR in L1210 cells with this product at 2 μg/ml.
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (N-Term) (ABIN501014) Western blot analysis of TOR in L1210 cell lysate with this product at (A) 1 and (B) ...