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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||226 Antibodies||85 Antibodies||78 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies||1 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies||11 Antibodies||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||65 Antibodies||31 Antibodies||29 Antibodies|
|Immunohistochemistry (IHC)||115 Antibodies||46 Antibodies|
|Antigen||Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MTOR antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MTOR AntibodyTarget Details MTOR Application Details Handling Images
|Specificity||This antibody detects endogenous levels of mTOR protein when phosphorylated at Ser2448.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details MTORProduct Details anti-MTOR Antibody Application Details Handling Images back to top
|Alternative Name||mTOR / FRAP1 (FRAP1 Antibody Abstract)|
|Background||MTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245- kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR|
|Molecular Weight||approx. 289 kDa|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MTOR Antibody Target Details MTOR Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MTOR Antibody Target Details MTOR Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
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