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|Antigen||Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MTOR antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-MTOR AntibodyTarget Details MTOR Application Details Handling Images
|Specificity||MTOR (P2476) antibody detects endogenous levels of mTOR protein.|
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details MTORProduct Details anti-MTOR Antibody Application Details Handling Images back to top
|Alternative Name||mTOR / FRAP1 (FRAP1 Antibody Abstract)|
|Background||Regulatory associated protein of mTOR, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (mTOR), and is essential for mTOR signalling in vivo. Raptor binding to mTOR is critical for mTORcatalysed substrate phosphorylation of 4EBP1. The Raptor-mTOR complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of mTOR kinase activity under nutrient-deprived conditions and is an important component in the mTOR pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events|
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ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MTOR Antibody Target Details MTOR Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
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