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anti-Rat (Rattus) MTOR antibody for Immunofluorescence

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • 2610315D21Rik
  • AI327068
  • flat
  • FRAP
  • frap1
  • Frap1
  • FRAP1
  • FRAP2
  • RAFT1
  • RAPT1
  • tor
  • wu:fc22h08
Epitope
AA 185-290
66
24
24
23
22
21
14
13
13
10
7
6
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
340
164
150
8
2
2
1
1
1
Host
Mouse
293
46
2
Clonality
Monoclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
221
112
92
63
51
44
32
20
8
8
6
3
2
2
2
2
2
1
Supplier
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Catalog No. ABIN968383
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32215732 ABIN1531447 IF IHC ELISA Rabbit IgG pSer2481 Log in to see Polyclonal
0.32215732 ABIN2705013 IC IF WB Rabbit C-Term Log in to see Polyclonal
0.32215732 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.32215732 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal
0.32215732 ABIN1110154 ICC IF IP Rabbit IgG Exon 5, Exon 6 Log in to see Polyclonal
0.001082034 ABIN196962 IF IHC (p) WB Rabbit pSer2448 Log in to see Polyclonal 4
0.001082034 ABIN968384 IF WB Mouse IgG1 AA 185-290 Log in to see 30-RAFT1 5
0.001082034 ABIN319329 IF IHC (p) WB Rabbit Ser2448 Log in to see Polyclonal 3
0.001082034 ABIN303334 EIA IF IHC (p) WB Rabbit AA 2440-2457 Log in to see Polyclonal
0.001082034 ABIN362868 IF IHC WB Rabbit IgG AA 2446-2450 Log in to see Polyclonal 1
0.001082034 ABIN362262 IF IHC Rabbit IgG pSer2448 Log in to see Polyclonal 3
0.001082034 ABIN498610 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.001082034 ABIN1876705 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.001082034 ABIN2470319 IF IHC ELISA WB Rabbit AA 1189-1199 Log in to see Polyclonal
0.001082034 ABIN1737123 IF IHC (p) ELISA WB Rabbit IgG AA 2440-2457 Log in to see Polyclonal
0.001082034 ABIN2755575 IC IF IP IHC WB Rabbit C-Term, pSer2448 Log in to see Polyclonal
0.001082034 ABIN3183004 ELISA IF IHC Rabbit IgG pSer2481 Log in to see Polyclonal
0.001082034 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Log in to see Polyclonal
0.001082034 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Log in to see Polyclonal
0.001082034 ABIN1842805 ICC IF IHC WB Rabbit pSer2481 Log in to see Polyclonal

Top referenced anti-MTOR antibody for Immunofluorescence

Similar anti-MTOR Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken
Western Blotting (WB) 225 Antibodies 85 Antibodies 79 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 37 Antibodies 12 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 65 Antibodies 31 Antibodies 30 Antibodies
Immunohistochemistry (IHC) 115 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope AA 185-290
(66), (24), (24), (23), (22), (21), (14), (13), (13), (10), (7), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(340), (164), (150), (8), (2), (2), (1), (1), (1)
Host Mouse
(293), (46), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(221), (112), (92), (63), (51), (44), (32), (20), (8), (8), (6), (3), (2), (2), (2), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN968383) Images
Cross-Reactivity Mouse (Murine), Human, Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat RAFT1 aa. 185-290
Clone 30-RAFT1
Isotype IgG1

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR Antibody (ABIN968383) Images back to top
Antigen
Alternative Name RAFT1 (FRAP1 Antibody Abstract)
Background Immunosuppressive agents such as cyclosporin A, FK506, and rapamycin are used routinely in clinical transplantation and the treatment of autoimmune disorders. Cyclosporin A and FK506 bind to cyclophilins and the FK-binding protein FKBP12, respectively. These complexes interact with, and inhibit the activity of, the calcineurin protein phosphatase, which is essential for T cell activation. Rapamycin also interacts with FKBP12, but this complex does not affect calcineurin. Instead, it inhibits signals required for G1 to S progression in yeast, in IL-2-stimulated T lymphocytes, and other mammalian cell types. Rapamycin-FKBP12 binds to a protein designated Rapamycin And FKBP12 Target-1 (RAFT1) in rats and FKBP-Rapamycin-Associated Protein (FRAP) in humans. RAFT1 is the homolog of the yeast genes TOR1 and TOR2 which, when mutated, lead to rapamycin resistance. RAFT1 is a PI4-kinase that exhibits homology with other PI kinases such as PI3-kinase and PI4-Kalpha. RAFT1 phosphorylates p70 S6 kinase and 4E-BP1 which are regulators of translational initiation. However, rapamycin-FKBP1 does not inhibit PI4-kinase activity and, therefore, affects RAFT1 activity via alternate mechanisms.
Synonyms: Rapamycin And FKBP12 Target-1
Molecular Weight 245 kDa
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR Antibody (ABIN968383) Images back to top
Comment

Related Products: ABIN968539, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR Antibody (ABIN968383) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-MTOR Antibody (ABIN968383)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Castedo, Ferri, Blanco, Roumier, Larochette, Barretina, Amendola, Nardacci, Métivier, Este, Piacentini, Kroemer: "Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/FKBP12-rapamycin-associated protein-mediated p53 phosphorylation." in: The Journal of experimental medicine, Vol. 194, Issue 8, pp. 1097-110, 2001

Alarcon, Cardenas, Heitman: "Mammalian RAFT1 kinase domain provides rapamycin-sensitive TOR function in yeast." in: Genes & development, Vol. 10, Issue 3, pp. 279-88, 1996

Sabatini, Pierchala, Barrow, Schell, Snyder: "The rapamycin and FKBP12 target (RAFT) displays phosphatidylinositol 4-kinase activity." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 20875-8, 1995

Background publications

Burnett, Barrow, Cohen, Snyder, Sabatini: "RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 4, pp. 1432-7, 1998

Sabatini, Erdjument-Bromage, Lui, Tempst, Snyder: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN968383) back to top
Supplier Images
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290) (ABIN968383) Western blot analysis of RAFT1 on a PC12 cell lysate (Rat neuroblastoma, ATCC CRL-172...
Immunofluorescence (IF) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 185-290) (ABIN968383) Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).