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anti-Rat (Rattus) MTOR antibody for Immunohistochemistry

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
pSer2448
61
26
26
21
21
21
15
15
12
10
9
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
346
196
194
18
9
8
7
7
7
7
4
2
2
2
1
1
Host
Rabbit
297
45
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
227
107
88
66
54
52
37
17
8
7
7
4
3
2
2
2
1
1
1
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Catalog No. ABIN1531910
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.142917 ABIN153493 ELISA EM IHC IHC (p) WB Rabbit IgG pSer2448 Log in to see Polyclonal 4
9.142917 ABIN447030 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
9.142917 ABIN1531447 IF IHC ELISA Rabbit IgG pSer2481 Log in to see Polyclonal
9.142917 ABIN1532942 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.142917 ABIN1532464 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.142917 ABIN2470319 IF IHC ELISA WB Rabbit AA 1189-1199 Log in to see Polyclonal
9.142917 ABIN2431685 IHC ELISA Rabbit IgG Log in to see Polyclonal
9.142917 ABIN2426243 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal 4
1 ABIN3183004 ELISA IF IHC Rabbit IgG pSer2481 Log in to see Polyclonal
1 ABIN3185708 ELISA IHC WB Rabbit IgG Ser394 Log in to see Polyclonal
1 ABIN3185707 ELISA IHC WB Rabbit IgG Ser392 Log in to see Polyclonal
1 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Log in to see Polyclonal
1 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Log in to see Polyclonal
1 ABIN362262 IF IHC Rabbit IgG pSer2448 Log in to see Polyclonal 3
1 ABIN362868 IF IHC WB Rabbit IgG AA 2446-2450 Log in to see Polyclonal 1
1 ABIN363086 IHC Rabbit IgG AA 2479-2483 Log in to see Polyclonal 3
1 ABIN966602 IHC Rabbit pSer2448 Log in to see 3
1 ABIN3198213 IHC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2755575 IC IF IP IHC WB Rabbit C-Term, pSer2448 Log in to see Polyclonal

Top referenced anti-MTOR antibody for Immunohistochemistry

Similar anti-MTOR Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 38 Antibodies 40 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 34 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 104 Antibodies
Flow Cytometry (FACS) 1 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope pSer2448
(61), (26), (26), (21), (21), (21), (15), (15), (12), (10), (9), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(346), (196), (194), (18), (9), (8), (7), (7), (7), (7), (4), (2), (2), (2), (1), (1)
Host Rabbit
(297), (45), (3), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(227), (107), (88), (66), (54), (52), (37), (17), (8), (7), (7), (4), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN1531910) Images
Specificity MTOR (Phospho-Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Ser2448.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2448.
Isotype IgG

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR Antibody (ABIN1531910) Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background Molecular weight of target protein: 288 kDa
Synonyms: FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein, kinase mTOR
Molecular Weight 288 kDa
Gene ID 2475
OMIM 601231
UniProt P42345
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, DNA/RNA
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR Antibody (ABIN1531910) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.338207 (NCBI Gene Symbol: MTOR)

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR Antibody (ABIN1531910) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-MTOR Antibody (ABIN1531910)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Luo, Yoneda, Ohmori, Sasaki, Shimbo, Eto, Kato, Miyano, Kobayashi, Sasahira, Chihara, Kuniyasu: "Cancer usurps skeletal muscle as an energy repository." in: Cancer research, Vol. 74, Issue 1, pp. 330-40, 2014

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN1531910) back to top
Supplier Images
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (pSer2448) (ABIN1531910) Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', usin...
Immunohistochemistry (IHC) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (pSer2448) (ABIN1531910) Immunohistochemistry analysis of paraffin-embedded human brain, using mTOR (Phospho-S...