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anti-Dog (Canine) PFKFB2 antibody for Western Blotting

Recommended PFKFB2 Antibody (supplied by: Log in to see )

Antigen
6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2) Antibodies
  • 4930568D07Rik
  • PFK-2/FBPase-2
  • pfkfb2
  • RH2K
  • zgc:56282
Epitope
N-Term
32
11
8
5
3
1
1
1
1
1
1
1
1
Reactivity
Human, Dog (Canine), Cow (Bovine)
86
44
29
6
4
2
1
Host
Goat
82
7
3
Clonality
Polyclonal
Conjugate
This PFKFB2 antibody is un-conjugated
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
68
39
33
13
11
4
3
3
1
1
Supplier
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Catalog No. ABIN570784
$ 221.76
Plus shipping costs $45.00

Similar anti-PFKFB2 Antibodies

Application / Reactivity Human Dog (Canine) Cow (Bovine)
Western Blotting (WB) 63 Antibodies 1 Antibodies 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
ELISA 40 Antibodies 5 Antibodies 5 Antibodies

General

Antigen 6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2) Antibodies
Epitope N-Term
(32), (11), (8), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Dog (Canine), Cow (Bovine)
(86), (44), (29), (6), (4), (2), (1)
Host Goat
(82), (7), (3)
Clonality
Polyclonal
Conjugate This PFKFB2 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(68), (39), (33), (13), (11), (4), (3), (3), (1), (1)
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Product Details anti-PFKFB2 Antibody

Target Details PFKFB2 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2816249
Specificity This antibody is expected to recognize both reported isoforms (NP_006203.2, NP_001018063.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence SEQNNNSYETKTP-C, from the N Terminus of the protein sequence according to NP_006203.2, NP_001018063.1.

Target Details PFKFB2

Product Details anti-PFKFB2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PFKFB2 (PFKFB2 Antibody Abstract)
Background Synonyms: PFKFB2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, DKFZp781D2217, MGC138308, MGC138310, PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme, OTTHUMP00000034285, OTTHUMP00000034286, PFKFB, cardiac, fructose-2,6-bisphosphatase, cardiac isozyme
Gene ID 5208
NCBI Accession NP_001018063, NP_006203
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-PFKFB2 Antibody Target Details PFKFB2 Handling Images back to top
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5 kDa according to NP_006203.2 and 54.4 kDa according to NP_001018063.1. The 75 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only

Handling

Product Details anti-PFKFB2 Antibody Target Details PFKFB2 Application Details Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.