PFKFB2 antibody (N-Term)

Catalog No. ABIN570784

This Goat Polyclonal antibody specifically detects PFKFB2 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for PFKFB2 antibody (N-Term) (ABIN570784)

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PFKFB2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PFKFB2 Antibody

Binding Specificity: N-Term

Purpose: PFKFB2

Sequence: SEQNNNSYET KTP

Specificity: This antibody is expected to recognize both reported isoforms (NP_006203.2, NP_001018063.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SEQNNNSYETKTP-C, from the N Terminus of the protein sequence according to NP_006203.2, NP_001018063.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5 kDa

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PFKFB2

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Alternative Name: PFKFB2

Background: PFKFB2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, DKFZp781D2217, MGC138308, MGC138310, PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme, OTTHUMP00000034285, OTTHUMP00000034286, P

Gene ID: 5208

NCBI Accession: NP_006203, NP_001018063

Pathways: PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process