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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Western Blotting (WB)||68 Antibodies||39 Antibodies||26 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies||10 Antibodies||9 Antibodies|
|Immunohistochemistry (IHC)||30 Antibodies||10 Antibodies||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies||13 Antibodies|
|Immunofluorescence (IF)||4 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Antigen||6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PFKFB2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PFKFB2 AntibodyTarget Details PFKFB2 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PFKFB2 protein. (region surrounding Pro479)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details PFKFB2Product Details anti-PFKFB2 Antibody Application Details Handling Images back to top
|Alternative Name||PFKFB2 (PFKFB2 Antibody Abstract)|
|Background||Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).Synonyms: 6-biphosphatase 2, 6-phosphofructo-2-kinase/fructose-2, PFK-2/FBPase-2, PFK/FBPase 2, PFKFB cardiac|
|Molecular Weight||approx. 58 kDa|
|Research Area||Cancer, Metabolism|
|Pathways||PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process|
Application DetailsProduct Details anti-PFKFB2 Antibody Target Details PFKFB2 Handling Images back to top
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PFKFB2 Antibody Target Details PFKFB2 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-PFKFB2 Antibody Target Details PFKFB2 Application Details Handling back to top