We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|BioImaging (BI)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||11 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||75 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||31 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||89 Antibodies|
|Antigen||Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies|
|Epitope||Middle Region Alternatives|
|Conjugate||This PIK3CA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-PIK3CA AntibodyTarget Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN360445) Images
|Specificity||This antibody reacts to PI3KCA.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the the center region of human PI3KCA.|
Target Details PIK3CAProduct Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN360445) Images back to top
|Alternative Name||PIK3CA (PIK3CA Antibody Abstract)|
|Background||Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.Synonyms: 5-bisphosphate 3-kinase catalytic subunit alpha isoform, EC=184.108.40.206, PI3-kinase p110 subunit alpha, PI3K, PIK3CA, Phosphatidylinositol-4, PtdIns-3-kinase p110|
|Gene ID||5290, 9606|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN360445) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN360445) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PIK3CA Antibody (ABIN360445)Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Broderick, Di, Parrett et al.: "Mutations of PIK3CA in anaplastic oligodendrogliomas, high-grade astrocytomas, and medulloblastomas." in: Cancer research, Vol. 64, Issue 15, pp. 5048-50, 2004 (PubMed).
Khan, Di Cello, Nath et al.: "Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells." in: Journal of neurovirology, Vol. 9, Issue 6, pp. 584-93, 2003 (PubMed).
Ballou, Lin, Fan et al.: "Activated G alpha q inhibits p110 alpha phosphatidylinositol 3-kinase and Akt." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23472-9, 2003 (PubMed).
Singh, Reddy, Goberdhan et al.: "p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas." in: Genes & development, Vol. 16, Issue 8, pp. 984-93, 2002 (PubMed).
ImagesProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN360445) back to top