anti-Human PIK3CA antibody for Enzyme Immunoassay

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Epitope
Tyr317
30
16
15
14
12
9
5
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
158
61
55
7
6
2
2
1
1
1
1
1
Host
Rabbit
110
30
21
1
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
7
6
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
100
80
68
36
15
13
10
9
6
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN360221
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1108651 EIA FACS IF WB Mouse IgG1 Log in to see 4F3 2
1 ABIN372794 EIA IHC (p) Goat IgG Internal Region Log in to see Polyclonal
1 ABIN5553650 EIA IHC (p) Goat Internal Region Log in to see Polyclonal

Top referenced anti-PIK3CA antibody for Enzyme Immunoassay

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Application / Reactivity Human
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ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 15 Antibodies
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Western Blotting (WB) 98 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope Tyr317
(30), (16), (15), (14), (12), (9), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(158), (61), (55), (7), (6), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(110), (30), (21), (1)
Clonality
Polyclonal
Conjugate This PIK3CA antibody is un-conjugated
(7), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(100), (80), (68), (36), (15), (13), (10), (9), (6), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling ProductDetails: References for anti-PIK3CA antibody (ABIN360221) Images
Specificity This antibody reacts to PK3CA.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y317 of human PK3CA.
Isotype Ig

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling ProductDetails: References for anti-PIK3CA antibody (ABIN360221) Images back to top
Antigen
Alternative Name PIK3CA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2.Synonyms: 5-bisphosphate 3-kinase catalytic subunit alpha isoform, EC=2.7.1.153, PI3-kinase p110 subunit alpha, PI3K, PIK3CA, Phosphatidylinositol-4, PtdIns-3-kinase p110
Gene ID 5290, 9606
UniProt P42336
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling ProductDetails: References for anti-PIK3CA antibody (ABIN360221) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details ProductDetails: References for anti-PIK3CA antibody (ABIN360221) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-PIK3CA antibody (ABIN360221)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Background publications

Zhao, Vogt: "Class I PI3K in oncogenic cellular transformation." in: Oncogene, Vol. 27, Issue 41, pp. 5486-96, 2008

Volinia, Hiles, Ormondroyd, Nizetic, Antonacci, Rocchi, Waterfield: "Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene." in: Genomics, Vol. 24, Issue 3, pp. 472-7, 1995

Hiles, Otsu, Volinia, Fry, Gout, Dhand, Panayotou, Ruiz-Larrea, Thompson, Totty: "Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit." in: Cell, Vol. 70, Issue 3, pp. 419-29, 1992

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling ProductDetails: References for anti-PIK3CA antibody (ABIN360221) back to top
Supplier Images
 image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (Tyr317) (ABIN360221) anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (Tyr317) antibody