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anti-Human PIK3CA antibody for Enzyme Immunoassay

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • 6330412C24Rik
  • C-P3K
  • caPI3K
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • p110
  • p110-alpha
  • p110alpha
  • pi3-k
  • PI3-kinase
  • PI3K
  • pi3k
  • PI3K-alpha
Epitope
Middle Region
34
16
14
14
13
9
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
146
50
43
7
4
2
1
1
1
Host
Rabbit
102
25
22
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
77
68
30
12
11
8
7
5
4
2
2
1
1
Supplier
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Catalog No. ABIN360445
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN360221 EIA WB Rabbit Ig Tyr317 Log in to see Polyclonal 3
0.0008870071 ABIN375147 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 1
0.0008870071 ABIN1108651 EIA FACS IF WB Mouse IgG1 Log in to see 4F3 2
0.0008870071 ABIN372794 EIA IHC (p) Goat IgG Internal Region Log in to see Polyclonal

Top referenced anti-PIK3CA antibody for Enzyme Immunoassay

Similar anti-PIK3CA Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
ELISA 69 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 78 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 89 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope Middle Region
(34), (16), (14), (14), (13), (9), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(146), (50), (43), (7), (4), (2), (1), (1), (1)
Host Rabbit
(102), (25), (22)
Clonality
Polyclonal
Conjugate This PIK3CA antibody is un-conjugated
(6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(90), (77), (68), (30), (12), (11), (8), (7), (5), (4), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN360445) Images
Specificity This antibody reacts to PI3KCA.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the the center region of human PI3KCA.
Isotype Ig

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN360445) Images back to top
Antigen
Alternative Name PIK3CA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.Synonyms: 5-bisphosphate 3-kinase catalytic subunit alpha isoform, EC=2.7.1.153, PI3-kinase p110 subunit alpha, PI3K, PIK3CA, Phosphatidylinositol-4, PtdIns-3-kinase p110
Gene ID 5290, 9606
UniProt P42336
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN360445) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN360445) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PIK3CA Antibody (ABIN360445)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Background publications

Broderick, Di, Parrett, Samuels, Cummins, McLendon, Fults, Velculescu, Bigner, Yan: "Mutations of PIK3CA in anaplastic oligodendrogliomas, high-grade astrocytomas, and medulloblastomas." in: Cancer research, Vol. 64, Issue 15, pp. 5048-50, 2004

Ballou, Lin, Fan, Jiang, Lin: "Activated G alpha q inhibits p110 alpha phosphatidylinositol 3-kinase and Akt." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23472-9, 2003

Khan, Di Cello, Nath, Kim: "Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells." in: Journal of neurovirology, Vol. 9, Issue 6, pp. 584-93, 2003

Singh, Reddy, Goberdhan, Walsh, Dao, Ngai, Chou, O-Charoenrat, Levine, Rao, Stoffel: "p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas." in: Genes & development, Vol. 16, Issue 8, pp. 984-93, 2002

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN360445) back to top
Supplier Images
 image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (Middle Region) (ABIN360445) anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (Middle Region) antibody
 image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (Middle Region) (ABIN360445) anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (Middle Region) antibody (Image 2)