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anti-Human PIK3CA antibody for ELISA

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • 6330412C24Rik
  • C-P3K
  • caPI3K
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • p110
  • p110-alpha
  • p110alpha
  • pi3-k
  • PI3-kinase
  • PI3K
  • pi3k
  • PI3K-alpha
Reactivity
Human
141
48
42
7
4
2
1
1
1
Host
Mouse
100
22
22
Clonality
Monoclonal
Conjugate
This PIK3CA antibody is un-conjugated
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
87
74
66
29
11
10
8
5
5
2
2
2
1
1
Supplier
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Catalog No. ABIN969555
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3112877 ABIN562228 PLA ELISA Mouse IgG1 kappa AA 959-1068, partial Log in to see 3G3
0.3112877 ABIN1844691 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.0012097829 ABIN334459 IHC ELISA WB Goat Internal Region Log in to see Polyclonal 2
0.0012097829 ABIN626004 IHC (p) IP ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012097829 ABIN337177 IHC (p) ELISA Goat Internal Region Log in to see Polyclonal
0.0012097829 ABIN1500200 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 4F3
0.0012097829 ABIN188684 IHC (p) IHC ELISA Goat IgG Internal Region Log in to see Polyclonal
0.0012097829 ABIN2466113 IHC ELISA Goat Internal Region Log in to see Polyclonal
0.0012097829 ABIN2492754 IHC (fp) ELISA Goat IgG Internal Region Log in to see Polyclonal
0.0012097829 ABIN221910 IP ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012097829 ABIN1953193 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Tyr317 Log in to see Polyclonal
0.0012097829 ABIN1954065 IHC ELISA WB APC Rabbit IgG Tyr317 Log in to see Polyclonal
0.0012097829 ABIN1956497 IHC ELISA WB FITC Rabbit IgG Tyr317 Log in to see Polyclonal
0.0012097829 ABIN1955315 IHC ELISA WB Biotin Rabbit IgG Tyr317 Log in to see Polyclonal
0.0012097829 ABIN1958838 IHC ELISA WB PE Rabbit IgG Tyr317 Log in to see Polyclonal
0.0012097829 ABIN1923919 IHC ELISA WB Biotin Rabbit IgG AA 511-541 Log in to see Polyclonal
0.0012097829 ABIN1923917 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 511-541 Log in to see Polyclonal
0.0012097829 ABIN1923920 IHC ELISA WB FITC Rabbit IgG AA 511-541 Log in to see Polyclonal
0.0012097829 ABIN1923921 IHC ELISA WB PE Rabbit IgG AA 511-541 Log in to see Polyclonal
0.0012097829 ABIN1923918 IHC ELISA WB APC Rabbit IgG AA 511-541 Log in to see Polyclonal

Top referenced anti-PIK3CA antibody for ELISA

Similar anti-PIK3CA Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
ELISA 68 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 86 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Reactivity Human
(141), (48), (42), (7), (4), (2), (1), (1), (1)
Host Mouse
(100), (22), (22)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PIK3CA antibody is un-conjugated
(6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(87), (74), (66), (29), (11), (10), (8), (5), (5), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN969555) Images
Purification Purified
Immunogen Purified recombinant fragment of human PIK3CA expressed in E. coli.
Clone 4F3
Isotype IgG1

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN969555) Images back to top
Antigen
Alternative Name PIK3CA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Molecular Weight 124 kDa
Gene ID 5290
HGNC 5290
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN969555) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN969555) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-PIK3CA Antibody (ABIN969555)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Edwards, Witherspoon, Wang, Afrasiabi, Pham, Birnbaumer, Lipkin: "Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease-associated colorectal cancer." in: Cancer research, Vol. 69, Issue 16, pp. 6423-9, 2009

Pang, Flinn, Patsialou, Wyckoff, Roussos, Wu, Pozzuto, Goswami, Condeelis, Bresnick, Segall, Backer: "Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase." in: Cancer research, Vol. 69, Issue 23, pp. 8868-76, 2009

Michelucci, Di Cristofano, Lami, Collecchi, Caligo, Decarli, Leopizzi, Aretini, Bertacca, Porta, Ricci, Della Rocca, Stanta, Bevilacqua, Cavazzana: "PIK3CA in breast carcinoma: a mutational analysis of sporadic and hereditary cases." in: Diagnostic molecular pathology : the American journal of surgical pathology, part B, Vol. 18, Issue 4, pp. 200-5, 2009

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN969555) back to top
Supplier Images
ELISA image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 2: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and...
Immunocytochemistry (ICC) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green) . ...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 4: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) ...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (ABIN969555) Figure 5: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 8...