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anti-Human PIK3CA antibody for Western Blotting

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • 6330412C24Rik
  • C-P3K
  • caPI3K
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • p110
  • p110-alpha
  • p110alpha
  • pi3-k
  • PI3-kinase
  • PI3K
  • pi3k
  • PI3K-alpha
Epitope
AA 504-533, Center
34
16
14
14
12
9
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
147
50
43
7
4
2
1
1
1
Host
Rabbit
102
25
23
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
78
69
30
12
11
8
7
5
5
2
2
1
1
Supplier
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Catalog No. ABIN392560
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN1882113 FACS IHC (p) WB Rabbit Ig AA 1019-1050, C-Term Log in to see Polyclonal 3
0.13341247 ABIN1108651 EIA FACS IF WB Mouse IgG1 Log in to see 4F3 2
0.13341247 ABIN967708 BI IP IHC WB Mouse IgG2a AA 562-724 Log in to see 4-PI3 5
0.13341247 ABIN967707 BI IP IHC WB Mouse IgG2a AA 562-724 Log in to see 4-PI3 5
0.13341247 ABIN968518 IF WB Mouse IgG1 AA 101-300 Log in to see 19-PI3 5
0.13341247 ABIN968519 IF WB Mouse IgG1 AA 101-300 Log in to see 19-PI3 5
0.13341247 ABIN969555 ICC FACS ELISA WB Mouse IgG1 Log in to see 4F3 3
0.13341247 ABIN3043462 WB Rabbit IgG AA 936-1068 Log in to see Polyclonal
0.13341247 ABIN2854758 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.13341247 ABIN498480 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN2706806 IHC WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN1874130 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1874129 WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN3020829 WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2492753 IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.13341247 ABIN3186429 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13341247 ABIN2403536 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2492755 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.13341247 ABIN1844691 ICC FACS IF ELISA WB Mouse IgG1 Log in to see
0.13341247 ABIN3016089 IP IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-PIK3CA antibody for Western Blotting

Similar anti-PIK3CA Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
ELISA 69 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 78 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 89 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope AA 504-533, Center
(34), (16), (14), (14), (12), (9), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(147), (50), (43), (7), (4), (2), (1), (1), (1)
Host Rabbit
(102), (25), (23)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PIK3CA antibody is un-conjugated
(6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(90), (78), (69), (30), (12), (11), (8), (7), (5), (5), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN392560) Images
Specificity This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 511~541 amino acids from the the center region of human PI3KCA.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 504-533 AA from the Central region of human PI3KCA.
Clone RB11623
Isotype Ig

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN392560) Images back to top
Antigen
Alternative Name PI3KCA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Synonyms: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, PI3K, PIK3CA,
Molecular Weight 124284 DA
Gene ID 5290
UniProt Q14CW1
Research Area Cancer, Autophagy, Cell Structure
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN392560) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN392560) Images back to top
Format Liquid
Concentration 1.57 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-PIK3CA Antibody (ABIN392560)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Background publications

Singh, Reddy, Goberdhan, Walsh, Dao, Ngai, Chou, O-Charoenrat, Levine, Rao, Stoffel: "p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas." in: Genes & development, Vol. 16, Issue 8, pp. 984-93, 2002

Shayesteh, Lu, Kuo, Baldocchi, Godfrey, Collins, Pinkel, Powell, Mills, Gray: "PIK3CA is implicated as an oncogene in ovarian cancer." in: Nature genetics, Vol. 21, Issue 1, pp. 99-102, 1999

Volinia, Hiles, Ormondroyd, Nizetic, Antonacci, Rocchi, Waterfield: "Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene." in: Genomics, Vol. 24, Issue 3, pp. 472-7, 1995

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN392560) back to top
Supplier Images
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 504-533) (ABIN392560) Western blot analysis of anti-PIK3CA Antibody (Center) Pab (ABIN392560) in K562 cell ...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 504-533) (ABIN392560) Western blot analysis of anti-PI3KCA Antibody (Center) Pab (ABIN392560) in 293 cell l...
Immunohistochemistry (IHC) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 504-533) (ABIN392560) Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antib...