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|Antigen||Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PIK3CA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-PIK3CA AntibodyTarget Details PIK3CA Application Details Handling Images
|Specificity||Recognizes PI 3 Kinase catalytic subunit alpha (PIK3CA).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide (C-HQWLKDKNKGEIYD) from an Internal region of Human PIK3CA|
Target Details PIK3CAProduct Details anti-PIK3CA Antibody Application Details Handling Images back to top
|Alternative Name||PIK3CA (PIK3CA Antibody Abstract)|
|Background||PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found, alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach.Synonyms: 5-bisphosphate 3-kinase catalytic subunit alpha isoform, EC=22.214.171.124, PI3-kinase p110 subunit alpha, PI3K, PIK3CA, Phosphatidylinositol-4, PtdIns-3-kinase p110|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-PIK3CA Antibody Target Details PIK3CA Handling Images back to top
ELISA: 1/128000. Immunohistochemistry on Paraffin Sections: 2.5-5.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Images back to top
|Buffer||Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ImagesProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling back to top