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|Antigen||Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies|
|Epitope||AA 1-30, N-Term Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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|Specificity||This antibody recognizes PPP2CA/PPP2CB.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide peptide between 1~30 amino acids from the N-terminal region of Human PPP2CA/B.|
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|Alternative Name||PPP2CA (PPP2CA Antibody Abstract)|
|Background||PPP2CA/B represents the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.Synonyms: PP2A-alpha, PP2A-beta, Protein Phospatase 2A, Replication protein C|
|Research Area||Kinases/Phosphatases, Signaling, Protein Modifications|
|Pathways||PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades|
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ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
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Gergs, Boknik, Buchwalow, Fabritz, Matus, Justus, Hanske, Schmitz, Neumann: "Overexpression of the catalytic subunit of protein phosphatase 2A impairs cardiac function." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40827-34, 2004
Prickett, Brautigan: "Overlapping binding sites in protein phosphatase 2A for association with regulatory A and alpha-4 (mTap42) subunits." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38912-20, 2004
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