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anti-Human Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Enzyme Immunoassay

Recommended Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
  • PP2A
  • Pp2a1
  • pp2ac
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • Ppp2ca
  • PPP2CA
  • R75353
  • RP-C
  • zgc:110641
Epitope
AA 1-30, N-Term
22
17
13
11
9
9
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
107
74
55
11
6
6
5
3
2
1
1
1
Host
Rabbit
101
15
4
3
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
103
44
26
25
15
12
11
6
2
1
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Catalog No. ABIN360806
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008858745 ABIN272071 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Enzyme Immunoassay

Similar anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibodies

Application / Reactivity Human
ELISA 31 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Western Blotting (WB) 92 Antibodies

General

Antigen Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
Epitope AA 1-30, N-Term
(22), (17), (13), (11), (9), (9), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(107), (74), (55), (11), (6), (6), (5), (3), (2), (1), (1), (1)
Host Rabbit
(101), (15), (4), (3), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(103), (44), (26), (25), (15), (12), (11), (6), (2), (1)
Pubmed 2 references available
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Product details

Target details Application Details Handling References Images
Specificity This antibody recognizes PPP2CA/PPP2CB.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide peptide between 1~30 amino acids from the N-terminal region of Human PPP2CA/B.
Isotype Ig

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name PPP2CA (PPP2CA Antibody Abstract)
Background PPP2CA/B represents the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.Synonyms: PP2A-alpha, PP2A-beta, Protein Phospatase 2A, Replication protein C
Gene ID 5515
NCBI Accession NP_002706
UniProt P67775
Research Area Kinases/Phosphatases, Signaling, Protein Modifications
Pathways PI3K-Akt Signaling

Application Details

Product details Target details Handling References Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References

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Background publications

Gergs, Boknik, Buchwalow, Fabritz, Matus, Justus, Hanske, Schmitz, Neumann: "Overexpression of the catalytic subunit of protein phosphatase 2A impairs cardiac function." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40827-34, 2004

Prickett, Brautigan: "Overlapping binding sites in protein phosphatase 2A for association with regulatory A and alpha-4 (mTap42) subunits." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38912-20, 2004

Images

Product details Target details Application Details Handling References back to top
Supplier Images
 image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-30), (N-Term) antibody (ABIN360806) anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-30), (N-Term) antibody
 image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-30), (N-Term) antibody (ABIN360806) anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-30), (N-Term) antibody (Image 2)