PPP2CA antibody

Catalog No. ABIN364316

The Mouse Monoclonal anti-PPP2CA antibody is suitable to detect PPP2CA in samples from Human. It has been validated for WB, IHC, ELISA, IHC (p) and IP.

Quick Overview for PPP2CA antibody (ABIN364316)

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP2CA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: P6C7

Product Details anti-PPP2CA Antibody

Purification: Protein G purified

Immunogen: Recombinant human PP2Ckappa protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (15 μg/mL), IP, WB (1:250 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Alternative Name: PP2Ac / PPP2CA

Background: Name/Gene ID: PPP2CA
Subfamily: PP2A
Family: Protein Phosphatase

Synonyms: PPP2CA, PP2Ac, PP2Calpha, ppp2A1, Protein phosphatase 2A alpha, Replication protein C, PP2CA, RP-C, PP2A-alpha

Gene ID: 5515

UniProt: P67775

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades