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anti-Human Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Immunofluorescence

Recommended Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
  • PP2A
  • Pp2a1
  • pp2ac
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • Ppp2ca
  • PPP2CA
  • R75353
  • RP-C
  • zgc:110641
Epitope
pTyr307
22
17
13
11
9
7
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
102
69
52
11
6
6
5
3
2
1
Host
Rabbit
99
11
4
3
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
98
41
23
23
13
11
11
5
2
2
Supplier
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Catalog No. ABIN1531377
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31815147 ABIN2856333 ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.31815147 ABIN497954 IF IHC (p) WB Rabbit AA 1-231, N-Term Log in to see Polyclonal
0.31815147 ABIN1532396 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.31815147 ABIN2499517 IF IHC ELISA WB Rabbit IgG Tyr307 Log in to see Polyclonal
0.0010831101 ABIN272071 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010831101 ABIN1108712 IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010831101 ABIN258417 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr307 Log in to see E155
0.0010831101 ABIN2888694 IF ELISA WB Rabbit IgG AA 260-309 Log in to see Polyclonal
0.0010831101 ABIN2891825 IF IHC WB Rabbit Thr301 Log in to see Polyclonal
0.0010831101 ABIN2957734 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.0010831101 ABIN408077 ICC IF IHC (p) IHC WB Rabbit IgG Thr301 Log in to see Polyclonal
0.0010831101 ABIN325661 IF IHC (p) Rat IgG2b AA 209-309 Log in to see
0.0010831101 ABIN441297 ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.0010831101 ABIN1578983 IF IHC ELISA WB Rabbit IgG Tyr307 Log in to see Polyclonal
0.0010831101 ABIN1578982 IF IHC ELISA WB Rabbit IgG pTyr307 Log in to see Polyclonal
0.0010831101 ABIN2969825 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 13 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 16 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 13 Antibodies
Immunoprecipitation (IP) 6 Antibodies 7 Antibodies
Western Blotting (WB) 42 Antibodies

General

Antigen Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
Epitope pTyr307
(22), (17), (13), (11), (9), (7), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(102), (69), (52), (11), (6), (6), (5), (3), (2), (1)
Host Rabbit
(99), (11), (4), (3), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(98), (41), (23), (23), (13), (11), (11), (5), (2), (2)
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Product details

Target details Application Details Handling Images
Specificity PP2A-alpha (Phospho-Tyr307) Antibody detects endogenous levels of PP2A-alpha only when phosphorylated at Tyr307.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307.
Isotype IgG

Target details

Product details Application Details Handling Images back to top
Antigen
Alternative Name PP2A-alpha (PPP2CA Antibody Abstract)
Background Molecular weight of target protein: 35 kDa
Synonyms: PP2A-alpha, PP2AA, RP-C, Replication protein C, Serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
Molecular Weight 35 kDa
Gene ID 5515
OMIM 176915
UniProt P67775
Pathways PI3K-Akt Signaling

Application Details

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Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.483408 (NCBI Gene Symbol: PPP2CA)

Restrictions For Research Use only

Handling

Product details Target details Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product details Target details Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (pTyr307) antibody (ABIN1531377) Western blot analysis of extracts from A549 cells, using PP2A-alpha (Phospho-Tyr307) ...
Immunohistochemistry (IHC) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (pTyr307) antibody (ABIN1531377) Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-a...
Immunofluorescence (IF) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (pTyr307) antibody (ABIN1531377) Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody...