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anti-Human Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Immunohistochemistry

Recommended Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
  • PP2A
  • Pp2a1
  • pp2ac
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • Ppp2ca
  • PPP2CA
  • R75353
  • RP-C
  • zgc:110641
Epitope
pTyr307
23
17
13
11
10
8
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
108
74
55
11
7
6
5
3
2
1
1
1
Host
Rabbit
101
15
4
4
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
104
44
26
25
14
12
11
6
2
2
Supplier
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Catalog No. ABIN1531377
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22865479 ABIN258417 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr307 Log in to see E155
0.22865479 ABIN1532396 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22865479 ABIN2499517 IF IHC ELISA WB Rabbit IgG Tyr307 Log in to see Polyclonal
0.22865479 ABIN2430653 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22865479 ABIN2423989 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN1585084 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0011340706 ABIN2891825 IF IHC WB Rabbit Thr301 Log in to see Polyclonal
0.0011340706 ABIN2957734 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011340706 ABIN2884262 ICC IHC ELISA WB Rabbit IgG pTyr307 Log in to see Polyclonal
0.0011340706 ABIN2886240 IHC Rabbit pTyr307 Log in to see Polyclonal
0.0011340706 ABIN408077 ICC IF IHC (p) IHC WB Rabbit IgG Thr301 Log in to see Polyclonal
0.0011340706 ABIN2339219 IHC WB Rabbit AA 6-20 Log in to see Polyclonal
0.0011340706 ABIN2339220 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.0011340706 ABIN1090916 IHC ELISA WB Rabbit IgG Catalytic Subunit, alpha Log in to see Polyclonal
0.0011340706 ABIN1090917 IHC ELISA WB Rabbit IgG Catalytic Subunit, alpha Log in to see Polyclonal
0.0011340706 ABIN441297 ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN2343111 IC IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.0011340706 ABIN2339221 IP IHC ELISA WB Mouse IgG2b kappa Log in to see P6C7
0.0011340706 ABIN2343105 IHC Rat IgG2b AA 209-309 Log in to see 8H124
0.0011340706 ABIN2564663 IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 14 Antibodies 28 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 6 Antibodies
Immunofluorescence (IF) 13 Antibodies 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 17 Antibodies 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies 16 Antibodies
Immunoprecipitation (IP) 6 Antibodies 8 Antibodies
Western Blotting (WB) 45 Antibodies

General

Antigen Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
Epitope pTyr307
(23), (17), (13), (11), (10), (8), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(108), (74), (55), (11), (7), (6), (5), (3), (2), (1), (1), (1)
Host Rabbit
(101), (15), (4), (4), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(104), (44), (26), (25), (14), (12), (11), (6), (2), (2)
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Product details

Target details Application Details Handling Images
Specificity PP2A-alpha (Phospho-Tyr307) Antibody detects endogenous levels of PP2A-alpha only when phosphorylated at Tyr307.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307.
Isotype IgG

Target details

Product details Application Details Handling Images back to top
Antigen
Alternative Name PP2A-alpha (PPP2CA Antibody Abstract)
Background Molecular weight of target protein: 35 kDa
Synonyms: PP2A-alpha, PP2AA, RP-C, Replication protein C, Serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
Molecular Weight 35 kDa
Gene ID 5515
OMIM 176915
UniProt P67775
Pathways PI3K-Akt Signaling

Application Details

Product details Target details Handling Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.483408 (NCBI Gene Symbol: PPP2CA)

Restrictions For Research Use only

Handling

Product details Target details Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product details Target details Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (pTyr307) antibody (ABIN1531377) Western blot analysis of extracts from A549 cells, using PP2A-alpha (Phospho-Tyr307) ...
Immunohistochemistry (IHC) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (pTyr307) antibody (ABIN1531377) Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-a...
Immunofluorescence (IF) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (pTyr307) antibody (ABIN1531377) Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody...