|Antigen||Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||This antibody detects endogenous levels of PP2A-alpha protein. (region surrounding Thr301)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen|
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|Alternative Name||PPP2CA (PPP2CA Antibody Abstract)|
|Background||In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.Synonyms: PP2A-alpha, Protein Phospatase 2A, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|
|Molecular Weight||approx. 35 kDa|
|Research Area||Kinases/Phosphatases, Signaling, Protein Modifications|
|Pathways||PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades|
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ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
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