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anti-Rat (Rattus) Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Enzyme Immunoassay

Recommended Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
  • PP2A
  • Pp2a1
  • pp2ac
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • Ppp2ca
  • PPP2CA
  • R75353
  • RP-C
  • zgc:110641
Reactivity
Human, Mouse (Murine), Rat (Rattus)
102
69
52
11
6
6
5
3
2
1
Host
Rabbit
99
11
4
3
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
98
42
24
22
13
11
11
5
2
1
Supplier
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Catalog No. ABIN272071
$ 390.50
Plus shipping costs $45.00

Similar anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 13 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 16 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 13 Antibodies
Immunoprecipitation (IP) 6 Antibodies 7 Antibodies
Western Blotting (WB) 42 Antibodies

General

Antigen Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(102), (69), (52), (11), (6), (6), (5), (3), (2), (1)
Host Rabbit
(99), (11), (4), (3), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(98), (42), (24), (22), (13), (11), (11), (5), (2), (1)
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Product details

Target details Application Details Handling Images
Specificity This antibody detects endogenous levels of PP2A-alpha protein. (region surrounding Thr301)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity Chromatography using epitope-specific immunogen

Target details

Product details Application Details Handling Images back to top
Antigen
Alternative Name PPP2CA (PPP2CA Antibody Abstract)
Background In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.Synonyms: PP2A-alpha, Protein Phospatase 2A, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Molecular Weight approx. 35 kDa
Gene ID 5515
NCBI Accession NP_002706
UniProt P67775
Research Area Kinases/Phosphatases, Signaling, Protein Modifications
Pathways PI3K-Akt Signaling

Application Details

Product details Target details Handling Images back to top
Application Notes ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product details Target details Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product details Target details Application Details Handling back to top
Supplier Images
 image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) antibody (ABIN272071) anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) antibody
 image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) antibody (ABIN272071) anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) antibody (Image 2)