anti-Cow (Bovine) RPS6KB1 antibody for ELISA

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • XPKAbeta
  • pkacb
  • 10539
  • 7084
  • CG10539
  • DS6K
  • Dmel\\CG10539
  • Dp70S6k
  • Dp70[s6k]
  • Dp70s6k
  • S6k
  • dS6K
  • dS6k
  • dp70[S6k]
  • dp70s6k
  • dps6k
  • ds6k
  • fs(3)07084
  • l(3)07084
  • p70 S6K
  • p70/S6K
  • p70S6K
  • p70[S6 kinase]
  • p70[S6K]
  • p70[S6k]
  • p70[S6kinase]
  • p70s6K
  • s6k
  • s6k11
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • p70s6k-A
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • protein kinase, cAMP-dependent, catalytic, beta
  • RPS6-p70-protein kinase
  • ribosomal protein S6 kinase, polypeptide 1
  • p70 S6 kinase
  • RPS6KB1
  • prkacb
  • S6k
  • Rps6kb1
  • p70s6k-A
Epitope
C-Term
100
36
30
28
24
23
20
20
20
19
18
18
17
16
15
15
15
14
14
11
11
8
7
6
6
6
5
5
5
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
689
445
422
72
53
41
34
33
21
13
13
8
5
5
3
3
1
Host
Rabbit
616
53
23
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
9
9
9
9
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
527
265
252
142
131
104
64
35
22
20
17
11
6
1
1
1
1
Options
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Catalog No. ABIN361700
$ 258.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2486881 ICC IF IP ELISA WB PerCP Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486869 ICC IF IP ELISA WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486876 ICC IF IP ELISA WB APC Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486879 ICC IF IP ELISA WB HRP Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486882 ICC IF IP ELISA WB PE Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486870 ICC IF IP ELISA WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486878 ICC IF IP ELISA WB FITC Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486868 ICC IF IP ELISA WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486883 ICC IF IP ELISA WB Streptavidin Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486867 ICC IF IP ELISA WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486871 ICC IF IP ELISA WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486872 ICC IF IP ELISA WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486873 ICC IF IP ELISA WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486874 ICC IF IP ELISA WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486877 ICC IF IP ELISA WB Biotin Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486875 ICC IF IP ELISA WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2486880 ICC IF IP ELISA WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN574364 IHC (p) ELISA WB Goat AA 490-502 Log in to see Polyclonal
1 ABIN597565 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN597566 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-RPS6KB1 antibody for ELISA

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 110 Antibodies 118 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 103 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 104 Antibodies
Immunohistochemistry (IHC) 156 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 82 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 292 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope C-Term
(100), (36), (30), (28), (24), (23), (20), (20), (20), (19), (18), (18), (17), (16), (15), (15), (15), (14), (14), (11), (11), (8), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(689), (445), (422), (72), (53), (41), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Host Rabbit
(616), (53), (23), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(527), (265), (252), (142), (131), (104), (64), (35), (22), (20), (17), (11), (6), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN361700) Images
Specificity Detects ~70 kDa.
Purification Protein A Purified
Immunogen Human p70 S6K c-terminal peptide -KLH conjugates

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN361700) Images back to top
Antigen
Alternative Name p70 S6K (RPS6KB1 Antibody Abstract)
Background Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70 % amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.
Gene ID 6199
NCBI Accession NP_003943
UniProt Q9UBS0
Research Area Kinases/Phosphatases, Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN361700) Images back to top
Application Notes
  • WB (1:1000)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

0.25 mg/ml was sufficient for detection of SPC-146 in lysates prepared from mouse muscle and mouse brain.

Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details ProductDetails: References for anti-RPS6KB1 antibody (ABIN361700) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

ProductDetails: References for anti-RPS6KB1 antibody (ABIN361700)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Background publications

Boyer, Quintanilla, Lee-Fruman: "Regulation of catalytic activity of S6 kinase 2 during cell cycle." in: Molecular and cellular biochemistry, Vol. 307, Issue 1-2, pp. 59-64, 2007

Valovka, Filonenko, Velykyï, Drobot, Voterfill, Matsuka, Hut: "[Features of fibronectin-dependent activation of ribosomal protein S6 kinase (S6K1 and S6K2)]." in: Ukrai?ns?kyi? biokhimichnyi? z?h?urnal (1999 ), Vol. 72, Issue 3, pp. 31-7, 2001

Shima, Pende, Chen, Fumagalli, Thomas, Kozma: "Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase." in: The EMBO journal, Vol. 17, Issue 22, pp. 6649-59, 1999

Lee-Fruman, Kuo, Lippincott, Terada, Blenis: "Characterization of S6K2, a novel kinase homologous to S6K1." in: Oncogene, Vol. 18, Issue 36, pp. 5108-14, 1999

Gout, Minami, Hara, Tsujishita, Filonenko, Waterfield, Yonezawa: "Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region." in: The Journal of biological chemistry, Vol. 273, Issue 46, pp. 30061-4, 1998

Kawasome, Papst, Webb, Keller, Johnson, Gelfand, Terada: "Targeted disruption of p70(s6k) defines its role in protein synthesis and rapamycin sensitivity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5033-8, 1998

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN361700) back to top
Supplier Images
 image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (C-Term) (ABIN361700) p70 S6K, mouse brain (R), neg CT (L)