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anti-Human RPS6KB1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • 70kDa
  • 2610318I15Rik
  • 4732464A07Rik
  • AA959758
  • AI256796
  • AI314060
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • p70/85s6k
  • p70S6K
  • p70s6k
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
Epitope
pSer417
109
35
31
29
24
24
21
19
18
18
18
17
16
15
14
13
13
13
12
11
9
8
8
7
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
649
359
344
48
30
21
16
15
6
1
1
1
1
Host
Rabbit
591
40
25
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
13
13
13
8
8
8
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
497
283
251
148
92
87
59
50
19
11
10
9
1
1
1
Supplier
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Catalog No. ABIN712976
$ 291.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008846439 ABIN873120 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
0.0008846439 ABIN713006 IF (p) IHC (p) WB Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
0.0008846439 ABIN712991 IF (p) IHC (p) WB Rabbit IgG pThr412 Log in to see Polyclonal
0.0008846439 ABIN2814986 IF (p) Alexa Fluor 594 Rabbit IgG AA 300-350 Log in to see Polyclonal
0.0008846439 ABIN876080 IF (p) Alexa Fluor 555 Rabbit IgG pSer417 Log in to see Polyclonal
0.0008846439 ABIN876083 IF (p) Alexa Fluor 488 Rabbit IgG pSer417 Log in to see Polyclonal
0.0008846439 ABIN876082 IF (p) Alexa Fluor 350 Rabbit IgG pSer417 Log in to see Polyclonal
0.0008846439 ABIN876081 IF (p) Alexa Fluor 647 Rabbit IgG pSer417 Log in to see Polyclonal
0.0008846439 ABIN876127 IF (p) Alexa Fluor 647 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
0.0008846439 ABIN876126 IF (p) Alexa Fluor 488 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
0.0008846439 ABIN876125 IF (p) Alexa Fluor 350 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
0.0008846439 ABIN876128 IF (p) Alexa Fluor 555 Rabbit IgG AA 420-440, pSer427 Log in to see Polyclonal
0.0008846439 ABIN914024 IF (p) Alexa Fluor 647 Rabbit IgG Log in to see Polyclonal
0.0008846439 ABIN914022 IF (p) Alexa Fluor 555 Rabbit IgG Log in to see Polyclonal
0.0008846439 ABIN914025 IF (p) Alexa Fluor 488 Rabbit IgG Log in to see Polyclonal
0.0008846439 ABIN914023 IF (p) Alexa Fluor 350 Rabbit IgG Log in to see Polyclonal
0.0008846439 ABIN712979 IF (p) Cy3 Rabbit IgG pSer417 Log in to see Polyclonal
0.0008846439 ABIN712981 IF (p) Cy5.5 Rabbit IgG pSer417 Log in to see Polyclonal
0.0008846439 ABIN712980 IF (p) Cy5 Rabbit IgG pSer417 Log in to see Polyclonal
0.0008846439 ABIN712982 IF (p) Cy7 Rabbit IgG pSer417 Log in to see Polyclonal

Top referenced anti-RPS6KB1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 95 Antibodies 98 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 11 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 34 Antibodies 35 Antibodies
Immunofluorescence (IF) 103 Antibodies 105 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 88 Antibodies 88 Antibodies
Immunohistochemistry (IHC) 153 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 35 Antibodies
Western Blotting (WB) 241 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope pSer417
(109), (35), (31), (29), (24), (24), (21), (19), (18), (18), (18), (17), (16), (15), (14), (13), (13), (13), (12), (11), (9), (8), (8), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(649), (359), (344), (48), (30), (21), (16), (15), (6), (1), (1), (1), (1)
Host Rabbit
(591), (40), (25), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (13), (13), (13), (8), (8), (8), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(497), (283), (251), (148), (92), (87), (59), (50), (19), (11), (10), (9), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN712976) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Ser417
Isotype IgG

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN712976) Images back to top
Antigen
Alternative Name Rps6kb1 (RPS6KB1 Antibody Abstract)
Background

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B.

Subcellular location: Cytoplasm, Nucleus


Synonyms: S6K, PS6K, S6K1, STK14A, p70-S6K, p70 S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, RPS6KB1
Gene ID 6198
UniProt P23443
Research Area Signaling, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN712976) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN712976) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-RPS6KB1 Antibody (ABIN712976)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Product cited in:

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014