You are viewing an incomplete version of our website. Please click to reload the website as full version.

Mouse (Murine) RPS6KB1 ELISA Kit

Recommended RPS6KB1 Product (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) ELISA Kits
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • p70S6K
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • ribosomal protein S6 kinase, polypeptide 1
  • RPS6KB1
  • Rps6kb1
Epitope
pSer424, pThr421, total
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
25
15
15
1
1
1
1
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN2748498
$ 628.10
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2748497 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN1380675 Cell ELISA Log in to see
1 ABIN1380238 Cell ELISA Log in to see
1 ABIN1380239 Cell ELISA Log in to see
1 ABIN1380241 Cell ELISA Log in to see
1 ABIN1380676 Cell ELISA Log in to see
1 ABIN1381391 Cell ELISA Log in to see
1 ABIN2114853 Cell ELISA Log in to see
1 ABIN2114854 Cell ELISA Log in to see
1 ABIN2114855 Cell ELISA Log in to see
1 ABIN2114857 Cell ELISA Log in to see
1 ABIN2114856 Cell ELISA Log in to see
1 ABIN2114852 Cell ELISA Log in to see
1 ABIN2685395 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN4991302 Sandwich ELISA Log in to see

Similar RPS6KB1 ELISA Kits

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
ELISA 26 ELISA Kits 16 ELISA Kits 16 ELISA Kits

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) ELISA Kits
Epitope pSer424, pThr421, total
Reactivity Human, Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
(25), (15), (15), (1), (1), (1), (1)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details RPS6KB1 ELISA Kit

Target details Application Details Handling Images
Purpose Human/Mouse/Rat Phospho-P70S6K (T421/S424) and Total P70S6K ELISA Kit. This assay semi-quantitatively measures phophorylated P70S6K (Thr421/S424) and Total P70S6K in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Detection Method Colorimetric
Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes human, mouse, and rat p70S6 Kinase phosphorylated at sites Threonine-421 and/or Serine-424 as well as total (pan) p70S6 Kinase.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details RPS6KB1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name P70S6K (RPS6KB1 ELISA Kit Abstract)
Background P70S6 kinase (P70S6K) phosphorylated at T421 and/or S424 and total (pan) p70S6 kinase
Gene ID 6198
UniProt P23443
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Smooth Muscle Cell Migration

Application Details

Product Details RPS6KB1 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details RPS6KB1 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months