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|Antigen||Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) ELISA Kits|
|Epitope||pSer424, pThr421, total|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Supplier||Log in to see|
Product Details RPS6KB1 ELISA KitTarget details Application Details Handling Images
|Purpose||Human/Mouse/Rat Phospho-P70S6K (T421/S424) and Total P70S6K ELISA Kit. This assay semi-quantitatively measures phophorylated P70S6K (Thr421/S424) and Total P70S6K in lysate samples.|
|Sample Type||Cell Lysate, Tissue Lysate|
|Specificity||The antibody pair provided in this kit recognizes human, mouse, and rat p70S6 Kinase phosphorylated at sites Threonine-421 and/or Serine-424 as well as total (pan) p70S6 Kinase.|
|Material not included||
Target detailsProduct Details RPS6KB1 ELISA Kit Application Details Handling Images back to top
|Alternative Name||P70S6K (RPS6KB1 ELISA Kit Abstract)|
|Background||P70S6 kinase (P70S6K) phosphorylated at T421 and/or S424 and total (pan) p70S6 kinase|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Smooth Muscle Cell Migration|
Application DetailsProduct Details RPS6KB1 ELISA Kit Target details Handling Images back to top
|Sample Volume||100 μL|
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
|Restrictions||For Research Use only|
HandlingProduct Details RPS6KB1 ELISA Kit Target details Application Details Images back to top
|Storage Comment||Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.|
|Expiry Date||6 months|