Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115), (full length) protein (His tag)

Details for Product No. ABIN1047347, Supplier: Log in to see
Protein Name
  • mif
  • Mif
  • gif
  • glif
  • mmif
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • GIF
  • Glif
  • GLIF
  • MMIF
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • macrophage migration inhibitory factor
  • Macrophage migration inhibitory factor
  • mif
  • MIF
  • Mif
  • PHATRDRAFT_49660
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • LOC100338701
Protein Characteristics
AA 2-115, full length
20
8
4
2
2
1
Origin
Human
55
16
3
1
1
1
Source
Escherichia coli (E. coli)
53
4
2
1
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This MIF protein is labelled with His tag.
Application
ELISA
Options
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Sequence PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA
Characteristics Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
Purity 90 %
Background Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Ref.23 Ref.25
Molecular Weight 16.4 kD
UniProt P14174
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
Comment

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions For Research Use only
Format Lyophilized
Concentration 0.2-2 mg/mL
Buffer Tris-based buffer, 50 % glycerol
Handling Advice Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
Storage -20 °C
Storage Comment Store at -20 °C for extended storage, conserve at -20 °C or -80 °C
Supplier Images
SDS-PAGE (SDS) image for Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115), (full length) protein (His tag) (ABIN1047347) Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-...
Background publications Mikayama, Nakano, Gomi, Nakagawa, Liu, Sato, Iwamatsu, Ishii, Weiser, Ishizaka: "Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 21, pp. 10056-60, 1993 (PubMed).

Weiser, Temple, Witek-Giannotti, Remold, Clark, David: "Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 19, pp. 7522-6, 1989 (PubMed).