PRMT1 Protein (AA 1-248, partial) (GST tag)

Catalog No. ABIN1047575

Product Details

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Protein Type: Recombinant

Protein Characteristics: AA 1-248, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PRMT1 protein is labelled with GST tag.

Application: SDS-PAGE (SDS), ELISA

Sequence: MAAAEAANCI MENFVATLAN GMSLQPPLEE VSCGQAESSE KPNAEDMTSK DYYFDSYAHF GIHEEMLKDE VRTLTYRNSM FHNRHLFKDK VVLDVGSGTG ILCMFAAKAG ARKVIGIECS SISDYAVKIV KANKLDHVVT IIKGKVEEVE LPVEKVDIII SEWMGYCLFY ESMLNTVLYA RDKWLAPDGL IFPDRATLYV TAIEDRQYKD YKIHWWENVY GFDMSCIKDV AIKEPLVDVV DPKQLVTN

Characteristics: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19

Purity: 90 %

Application Details

Comment:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 6M guanidine hydrochloride, 20mM Tris

Storage: -20 °C

References

Scorilas, Black, Talieri, Diamandis: "Genomic organization, physical mapping, and expression analysis of the human protein arginine methyltransferase 1 gene." in: Biochemical and biophysical research communications, Vol. 278, Issue 2, pp. 349-59, (2000) (PubMed).

Scott, Antonarakis, Lalioti, Rossier, Silver, Henry: "Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2)." in: Genomics, Vol. 48, Issue 3, pp. 330-40, (1998) (PubMed).

Target Details

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Alternative Name: Protein arginine N-methyltransferase 1 (PRMT1 Products)

Synonyms: ANM1 Protein, HCP1 Protein, HRMT1L2 Protein, IR1B4 Protein, 6720434D09Rik Protein, AW214366 Protein, Hrmt1l2 Protein, Mrmt1 Protein, 3E10 Protein, anm1 Protein, hcp1 Protein, hrmt1l2 Protein, ir1b4 Protein, prmt1 Protein, prmt1b Protein, xPRMT1b Protein, xprmt1 Protein, xPRMT1 Protein, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 1A Protein, ATPRMT1A Protein, F6F22.30 Protein, F6F22_30 Protein, protein arginine methyltransferase 1A Protein, DDBDRAFT_0183976 Protein, DDBDRAFT_0235399 Protein, DDB_0183976 Protein, DDB_0235399 Protein, PRMT1 Protein, DKFZp459J1326 Protein, fb39h07 Protein, wu:fb39h07 Protein, zf1 Protein, zgc:66201 Protein, protein arginine methyltransferase 1 Protein, protein arginine N-methyltransferase 1 Protein, protein arginine methyltransferase 1 L homeolog Protein, protein arginine methyltransferase 1 S homeolog Protein, protein arginine methyltransferase 1A Protein, protein arginine N-methyltransferase-1 Protein, protein arginine N-methyltransferase Protein, protein arginine methyltransferase Protein, PRMT1 Protein, Prmt1 Protein, prmt1.L Protein, prmt1.S Protein, PRMT1A Protein, prm-1 Protein, EDI_007800 Protein, Smp_029240.3 Protein, prmt1 Protein

Background: Synonyms: Histone-arginine N-methyltransferase PRMT1,Interferon receptor 1-bound protein 4

Molecular Weight: 55.4 kD

UniProt: Q99873