ATG3 Protein (AA 1-287) (His tag,T7 tag)

Catalog No. ABIN1096065

Product Details

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Protein Type: Recombinant

Protein Characteristics: AA 1-287

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ATG3 protein is labelled with His tag,T7 tag.

Purpose: Recombinant Human Autophagy Related 3 Homolog/ATG3 (C-6His, N-T7 tag)

Sequence: GSMQNVINTV KGKALEVAEY LTPVLKESKF KETGVITPEE FVAAGDHLVH HCPTWQWATG EELKVKAYLP TGKQFLVTKN VPCYKRCKQM EYSDELEAII EEDDGDGGWV DTYHNTGITG ITEAVKEITL ENKDNIRLQD CSALCEEEED EDEGEAADME EYEESGLLET DEATLDTRKI VEACKAKTDA GGEDAILQTR TYDLYITYDK YYQTPRLWLF GYDEQRQPLT VEHMYEDISQ DHVKKTVTIE NHPHLPPPPM CSVHPCRHAE VMKKIIETVA EGGGELGVHM YLLIFLKFVQ AVIPTIEYDY TRHFTM

Characteristics:

Recombinant Human Autophagy Related 3 Homolog/ATG3 (C-6His, N-T7 tag)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: Ubiquitin-Like-Conjugating Enzyme ATG3/ATG3 (ATG3 Products)

Synonyms: atg3 Protein, im:6910145 Protein, wu:fb15g12 Protein, zgc:64156 Protein, APG3 Protein, APG3-LIKE Protein, APG3L Protein, PC3-96 Protein, 2610016C12Rik Protein, Apg3l Protein, Atg3l Protein, PIG-1 Protein, Pig1 Protein, ATATG3 Protein, K11J9.1 Protein, K11J9_1 Protein, autophagy related 3 Protein, autophagy 3 (APG3) Protein, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy Protein, atg3 Protein, ATG3 Protein, Atg3 Protein

Background: Recombinant Human Ubiquitin-Like-Conjugating Enzyme ATG3/ATG3 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-His287) of Human ATG3 fused with a 6His tag at the C-terminus.
Ubiquitin-Like-Conjugating Enzyme ATG3 (ATG3) is widely expressed and has highly levels in heart, skeletal muscle, kidney, liver and placenta. ATG3 as a E2-like enzyme, involves in autophagy and mitochondrial homeostasis. ATG3 catalyzes the conjugation of ATG8-like proteins to PE which is essential for autophagy. As an autocatalytic E2-like enzyme, ATG3 also can catalyzes the conjugation of ATG12 to itself which palys a role in mitochondrial homeostasis but not in autophagy.

Molecular Weight: 36 kDa

UniProt: Q9NT62

Pathways: Autophagy