ATG3 Protein (AA 1-314) (His tag)
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- Target See all ATG3 Proteins
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-314
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ATG3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMQNVINT VKGKALEVAE YLTPVLKESK FKETGVITPE EFVAAGDHLV HHCPTWQWAT GEELKVKAYL PTGKQFLVTK NVPCYKRCKQ MEYSDELEAI IEEDDGDGGW VDTYHNTGIT GITEAVKEIT LENKDNIRLQ DCSALCEEEE DEDEGEAADM EEYEESGLLE TDEATLDTRK IVEACKAKTD AGGEDAILQT RTYDLYITYD KYYQTPRLWL FGYDEQRQPL TVEHMYEDIS QDHVKKTVTI ENHPHLPPPP MCSVHPCRHA EVMKKIIETV AEGGGELGVH MYLLIFLKFV QAVIPTIEYD YTRHFTM
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product ATG3 Protein
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ATG3 Autophagy Related 3 (ATG3) (AA 1-287) protein (His tag)
ATG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (GST tag)ATG3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATG3 Autophagy Related 3 (ATG3) (AA 1-100) protein (GST tag)ATG3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (Strep Tag)Crystallography grade ATG3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (Strep Tag)Crystallography grade ATG3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314), (full length) protein (His-SUMO Tag)ATG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
ATG3 Autophagy Related 3 (ATG3) protein (Myc-DYKDDDDK Tag)ATG3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- Alternative Name
- ATG3 (ATG3 Products)
- Synonyms
- atg3 Protein, im:6910145 Protein, wu:fb15g12 Protein, zgc:64156 Protein, APG3 Protein, APG3-LIKE Protein, APG3L Protein, PC3-96 Protein, 2610016C12Rik Protein, Apg3l Protein, Atg3l Protein, PIG-1 Protein, Pig1 Protein, ATATG3 Protein, K11J9.1 Protein, K11J9_1 Protein, autophagy related 3 Protein, autophagy 3 (APG3) Protein, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy Protein, atg3 Protein, ATG3 Protein, Atg3 Protein
- Background
- Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy. Recombinant human ATG3 protien, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 38.3 kDa (337aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_071933
- UniProt
- Q9NT62
- Pathways
- Autophagy
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