UPB1 Protein (AA 1-384) (His tag)

Catalog No. ABIN1096154

Product Details

Target: UPB1 (Ureidopropionase, beta (UPB1))

Protein Type: Recombinant

Protein Characteristics: AA 1-384

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UPB1 protein is labelled with His tag.

Purpose: Recombinant Human β-Ureidopropionase/UPB1 (C-6His)

Sequence: MAGAEWKSLE ECLEKHLPLP DLQEVKRVLY GKELRKLDLP REAFEAASRE DFELQGYAFE AAEEQLRRPR IVHVGLVQNR IPLPANAPVA EQVSALHRRI KAIVEVAAMC GVNIICFQEA WTMPFAFCTR EKLPWTEFAE SAEDGPTTRF CQKLAKNHDM VVVSPILERD SEHGDVLWNT AVVISNSGAV LGKTRKNHIP RVGDFNESTY YMEGNLGHPV FQTQFGRIAV NICYGRHHPL NWLMYSINGA EIIFNPSATI GALSESLWPI EARNAAIANH CFTCAINRVG TEHFPNEFTS GDGKKAHQDF GYFYGSSYVA APDSSRTPGL SRSRDGLLVA KLDLNLCQQV NDVWNFKMTG RYEMYARELA EAVKSNYSPT IVKEVEHHHH HH

Characteristics: Recombinant Human beta-Ureidopropionase/UPB1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Glu384) of Human UPB1 fused with a His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UPB1 (Ureidopropionase, beta (UPB1))

Alternative Name: BUP-1 (UPB1 Products)

Synonyms: MGC82230 Protein, wu:fb69e03 Protein, zgc:64020 Protein, BUP1 Protein, AI195023 Protein, Bup1 Protein, ureidopropionase, beta S homeolog Protein, beta-ureidopropionase 1 Protein, ureidopropionase, beta Protein, UreidoPropionase Beta Protein, upb1.S Protein, UPB1 Protein, upb1 Protein, upb-1 Protein, Upb1 Protein

Sub Type: Fusionprotein

Background: Beta-Ureidopropionase is a cytoplasmic protein which belongs to the CN hydrolase family of BUP subfamily. beta-Ureidopropionase binds one zinc ion per subunit, catalyzes the last step in the pyrimidine degradation pathway. beta-Ureidopropionase can convert N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to beta-aminoisobutyric acid and beta-alanine, ammonia and carbon dioxide, respectively. The pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta aminoisobutyric acid, respectively. Defects in beta-Ureidopropionase are the cause of beta-Ureidopropionase deficiency that is characterized by muscular hypotonia, dystonic movements, scoliosis, microcephaly and severe developmental delay.
Alternative Names: Beta-Ureidopropionase, BUP-1, Beta-Alanine Synthase, N-Carbamoyl-Beta-Alanine Amidohydrolase, UPB1, BUP1

Molecular Weight: 44.22 kDa

UniProt: Q9UBR1