Cytochrome b5 (CYTB5) (AA 2-134) protein (His tag)
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- Target See all Cytochrome b5 (CYTB5) Proteins
- Cytochrome b5 (CYTB5)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-134
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human Cytochrome b5/CYB5A (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAEQSDEAVK YYTLEEIQKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT ENFEDVGHST DAREMSKTFI IGELHPDDRP KLNKPPETLI TTIDSSSSWW TNWVIPAISA VAVALMYRLY MAED
- Characteristics
- Recombinant Human Cytochrome b5/CYB5A is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Asp134) of Human CYB5A fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CYTB5 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 0.1 mM EDTA, pH 7.25.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- Cytochrome b5 (CYTB5)
- Alternative Name
- Cytochrome-b5 (CYTB5 Products)
- Synonyms
- CYB5 Protein, MCB5 Protein, Cyb5 Protein, 0610009N12Rik Protein, Cyb5a Protein, CYB5A Protein, 1857 Protein, BcDNA:GH02503 Protein, BcDNA:RE66521 Protein, CG2140 Protein, CYT-B5 Protein, CYTB5 Protein, Dmel\\CG2140 Protein, dpld Protein, l(2)01857 Protein, l(2)k00107 Protein, cytochrome b5 type A Protein, cytochrome b5 type A (microsomal) Protein, cytochrome b5 Protein, Cytochrome b5 Protein, CYB5A Protein, Cyb5a Protein, LOC100328915 Protein, LOC100735904 Protein, Cyt-b5 Protein
- Sub Type
- Fusionprotein
- Background
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Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.
Alternative Names: Cytochrome b5, Microsomal Cytochrome b5 Type A, MCB5, CYB5A, CYB5 - Molecular Weight
- 17.5 kDa
- UniProt
- P00167
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