LSM4 Protein (AA 1-139) (His tag)
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- Target See all LSM4 Proteins
- LSM4 (LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-139
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LSM4 protein is labelled with His tag.
- Purpose
- Recombinant Human U6 snRNA-Associated Sm-Like Protein LSm4/LSM4 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ
- Characteristics
- Recombinant Human U6 snRNA-Associated Sm-Like Protein LSm4/LSM4 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Gln139) of Human LSM4 fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product LSM4 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, pH 8.0 .
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- LSM4 (LSM4 Homolog, U6 Small Nuclear RNA Associated (LSM4))
- Alternative Name
- U6 SnRNA-Associated Sm-Like Protein LSm4 (LSM4 Products)
- Synonyms
- GRP Protein, YER112W Protein, LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated S homeolog Protein, LSM4 homolog, U6 small nuclear RNA and mRNA degradation associated Protein, lsm4.S Protein, LSM4 Protein, Lsm4 Protein
- Sub Type
- Fusionprotein
- Background
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U6 snRNA-associated Sm-like protein LSm4 (LSM4) is a member of the snRNP Sm proteins family. Sm-like proteins contain the Sm sequence motif and are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM4 forms a heteromer with a donut shape. The complexes are involved in various steps of RNA metabolism. LSM4 binds specifically to the 3-terminal U-tract of U6 snRNA. LSM4 contributes RNA protein interactions and structural changes which are essential during ribosomal subunit assembly.
Alternative Names: U6 snRNA-Associated Sm-Like Protein LSm4, Glycine-Rich Protein, GRP, LSM4 - Molecular Weight
- 17.5 kDa
- UniProt
- Q9Y4Z0
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