IL-33 Protein (AA 112-270) (His tag)

Catalog No. ABIN1096945

Product Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Protein Type: Recombinant

Protein Characteristics: AA 112-270

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IL-33 protein is labelled with His tag.

Purpose: Recombinant Human Interleukin-33/IL-33 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET

Characteristics: Recombinant Human Interleukin-33/IL-33 (HIS tagged) is produced by our E. coli expression system. The target protein is expressed with sequence (Ser112-Thr270) of Human IL-33 fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.25.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: Interleukin-33/IL-33 (IL33 Products)

Synonyms: IL33 Protein, C9orf26 Protein, DVS27 Protein, IL1F11 Protein, NF-HEV Protein, NFEHEV Protein, RP11-575C20.2 Protein, 9230117N10Rik Protein, Il-33 Protein, Il1f11 Protein, RGD1311155 Protein, interleukin 33 Protein, IL33 Protein, Il33 Protein

Sub Type: Fusionprotein

Background: Interleukin-33 (IL-33) belongs to the IL-1 superfamily. IL33 is highly expressed in endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes, but almost undetectable in placenta. IL33 induces the production of T helper-2 (Th2)-associated cytokines. IL-33 is a cytokine that mediates its biological effects by binding to and signals through IL1RL1/ST2 and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-kappaB and MAP kinase signaling pathways that drive production of type 2 cytokines from polarized Th2 cells.
Alternative Names: Interleukin-33, IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Factor From High Endothelial Venules, NF-HEV, IL33, C9orf26, IL1F11, NFHEV

Molecular Weight: 20.3 kDa

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response