Lactate Dehydrogenase A Protein (LDHA) (AA 2-332) (His tag)
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- Target See all Lactate Dehydrogenase A (LDHA) Proteins
- Lactate Dehydrogenase A (LDHA)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-332
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Lactate Dehydrogenase A protein is labelled with His tag.
- Purpose
- Recombinant Human L-Lactate Dehydrogenase A Chain/LDH-A/PIG19 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
- Characteristics
- Recombinant Human L-Lactate Dehydrogenase A Chain/LDH-A is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Phe332) of Human LDHA fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product LDHA Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 50 % Glycerol, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- Lactate Dehydrogenase A (LDHA)
- Alternative Name
- LDH-A (LDHA Products)
- Synonyms
- GSD11 Protein, LDH1 Protein, LDHM Protein, LDH-M Protein, ldh-h Protein, ldha Protein, ldha-B Protein, ldhbb Protein, trg-5 Protein, Ldh1 Protein, Ldhm Protein, l7R2 Protein, gsd11 Protein, ldh-m Protein, ldh1 Protein, ldhm Protein, pig19 Protein, ldha1 Protein, ldhab Protein, LDH-A Protein, LDHC Protein, lactate dehydrogenase A Protein, lactate dehydrogenase B L homeolog Protein, lactate dehydrogenase A4 Protein, lactate dehydrogenase A L homeolog Protein, L-lactate dehydrogenase A chain Protein, LDHA Protein, ldhb.L Protein, Ldha Protein, ldha Protein, ldha.L Protein, LOC100713875 Protein
- Sub Type
- Fusionprotein
- Background
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L-Lactate Dehydrogenase A Chain (LDHA) is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA contains an N-terminal coenzyme binding region, a central catalytic site, and at least nine utilized Lys acetylation and two Tyr phosphorylation sites. LDHA belongs to the lactate dehydrogenase family, expressed predominantly in muscle tissue. LDHA mutations have been linked to exertional myoglobinuria.
Alternative Names: L-Lactate Dehydrogenase A Chain, LDH-A, Cell Proliferation-Inducing Gene 19 Protein, LDH Muscle Subunit, LDH-M, Renal Carcinoma Antigen NY-REN-59, LDHA - Molecular Weight
- 38.8 kDa
- UniProt
- P00338
- Pathways
- Warburg Effect
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