LDLR Protein (AA 22-788) (His tag)

Catalog No. ABIN1097071

Product Details

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Protein Type: Recombinant

Protein Characteristics: AA 22-788

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This LDLR protein is labelled with His tag.

Purpose: Recombinant Human Low-Density Lipoprotein Receptor/LDLR (C-6His)

Sequence: AVGDRCERNE FQCQDGKCIS YKWVCDGSAE CQDGSDESQE TCLSVTCKSG DFSCGGRVNR CIPQFWRCDG QVDCDNGSDE QGCPPKTCSQ DEFRCHDGKC ISRQFVCDSD RDCLDGSDEA SCPVLTCGPA SFQCNSSTCI PQLWACDNDP DCEDGSDEWP QRCRGLYVFQ GDSSPCSAFE FHCLSGECIH SSWRCDGGPD CKDKSDEENC AVATCRPDEF QCSDGNCIHG SRQCDREYDC KDMSDEVGCV NVTLCEGPNK FKCHSGECIT LDKVCNMARD CRDWSDEPIK ECGTNECLDN NGGCSHVCND LKIGYECLCP DGFQLVAQRR CEDIDECQDP DTCSQLCVNL EGGYKCQCEE GFQLDPHTKA CKAVGSIAYL FFTNRHEVRK MTLDRSEYTS LIPNLRNVVA LDTEVASNRI YWSDLSQRMI CSTQLDRAHG VSSYDTVISR DIQAPDGLAV DWIHSNIYWT DSVLGTVSVA DTKGVKRKTL FRENGSKPRA IVVDPVHGFM YWTDWGTPAK IKKGGLNGVD IYSLVTENIQ WPNGITLDLL SGRLYWVDSK LHSISSIDVN GGNRKTILED EKRLAHPFSL AVFEDKVFWT DIINEAIFSA NRLTGSDVNL LAENLLSPED MVLFHNLTQP RGVNWCERTT LSNGGCQYLC LPAPQINPHS PKFTCACPDG MLLARDMRSC LTEAEAAVAT QETSTVRLKV SSTAVRTQHT TTRPVPDTSR LPGATPGLTT VEIVTMSHQA LGDVAGRGNE KKPSSVRLEN LYFQGHHHHH H

Characteristics: Recombinant Human Low-Density Lipoprotein Receptor/LDLR is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala22-Arg788) of Human LDLR fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Alternative Name: LDL-receptor (LDLR Products)

Synonyms: FH Protein, FHC Protein, LDLCQ2 Protein, Hlb301 Protein, LDLRA Protein, LDLA Protein, LDL receptor-2 Protein, fhc Protein, ldlcq2 Protein, ldlr Protein, ldlr2-a Protein, LDL receptor 1 Protein, ldlr-a Protein, ldlr-b Protein, low density lipoprotein receptor Protein, low density lipoprotein receptor a Protein, low density lipoprotein receptor S homeolog Protein, low density lipoprotein receptor L homeolog Protein, LDLR Protein, Ldlr Protein, ldlra Protein, ldlr.S Protein, ldlr.L Protein

Sub Type: Fusionprotein

Background: Low-Density Lipoprotein Receptor (LDLR) is a transmembrane glycoprotein that plays a critical role in cholesterol homeostasis. LDLR mediates blood cholesterol level by interacting with lipoprotein particles like LDL and VLDL. The extracellular domain of LDLR contains LDL receptor type A (ligand-binding) modules (LA repeats), epidermal growth factor-like modules, and LY repeats containing the YWTD consensus motif that are important in binding and releasing of ApoB-100 and ApoE in lipoprotein particles. The C terminal domain of LDLR inside the cell is required for the receptor internalization. Loss of function mutations in the LDLR gene causes Familial Hypercholesterolemia (FH).
Alternative Names: Low-Density Lipoprotein Receptor, LDL Receptor, LDLR

Molecular Weight: 84.78 kDa

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism