UBE2S Protein (AA 1-222) (GST tag)

Catalog No. ABIN1097736

Product Details

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Protein Type: Recombinant

Protein Characteristics: AA 1-222

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2S protein is labelled with GST tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 S/UBE2S (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 S/UBE2S is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu222) of Human UBE2S fused with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Alternative Name: Ubiquitin-Conjugating Enzyme E2 S/UBE2S (UBE2S Products)

Synonyms: E2-EPF Protein, E2EPF Protein, EPF5 Protein, 0910001J09Rik Protein, 6720465F12Rik Protein, AA409170 Protein, RGD1564746 Protein, ube2s Protein, UBE2S Protein, DDBDRAFT_0188215 Protein, DDBDRAFT_0305006 Protein, DDB_0188215 Protein, DDB_0305006 Protein, e2-epf Protein, e2epf Protein, epf5 Protein, ube2s.1 Protein, ube2s.1-b Protein, ube2s.1-a Protein, ubiquitin conjugating enzyme E2 S Protein, ubiquitin-conjugating enzyme E2S Protein, ubiquitin-conjugating enzyme e2S, putative Protein, ubiquitin-conjugating enzyme E2 S Protein, ubiquitin conjugating enzyme E2 S S homeolog Protein, ubiquitin conjugating enzyme E2 S L homeolog Protein, UBE2S Protein, Ube2s Protein, ube2s Protein, ATEG_00324 Protein, Smp_180170 Protein, MCYG_01532 Protein, ube2s.S Protein, ube2s.L Protein

Sub Type: Fusionprotein

Background: Ubiquitin-Conjugating Enzyme E2 S (UBE2S) is a member of the Ubiquitin-Conjugating Enzyme family. UBE2S interacts with CDC20, FZR1/CDH1 and VHL. UBE2S can form a thiol ester linkage with Ubiquitin in an Ubiquitin Activating Enzyme-Dependent manner, a characteristic property of Ubiquitin Carrier Proteins. UBE2S acts as an essential factor of the Anaphase Promoting Complex/Cyclosome, a cell cycle-regulated Ubiquitin ligase that controls progression through mitosis. UBE2S is also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 S, E2-EPF, Ubiquitin Carrier Protein S, Ubiquitin-Conjugating Enzyme E2-24 kDa, Ubiquitin-Conjugating Enzyme E2-EPF5, Ubiquitin-Protein Ligase S, UBE2S, E2EPF

Molecular Weight: 50.13 kDa

UniProt: Q16763

Pathways: Ubiquitin Proteasome Pathway