UBE2S Protein (GST tag)

Catalog No. ABIN7319153

Product Details

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2S protein is labelled with GST tag.

Purpose: Recombinant Human UBE2S Protein (GST Tag)

Sequence: Met 1-Leu222

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 S is produced by our E.coli expression system and the target gene encoding Met1-Leu222 is expressed with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Alternative Name: UBE2S (UBE2S Products)

Synonyms: E2-EPF Protein, E2EPF Protein, EPF5 Protein, 0910001J09Rik Protein, 6720465F12Rik Protein, AA409170 Protein, RGD1564746 Protein, ube2s Protein, UBE2S Protein, DDBDRAFT_0188215 Protein, DDBDRAFT_0305006 Protein, DDB_0188215 Protein, DDB_0305006 Protein, e2-epf Protein, e2epf Protein, epf5 Protein, ube2s.1 Protein, ube2s.1-b Protein, ube2s.1-a Protein, ubiquitin conjugating enzyme E2 S Protein, ubiquitin-conjugating enzyme E2S Protein, ubiquitin-conjugating enzyme e2S, putative Protein, ubiquitin-conjugating enzyme E2 S Protein, ubiquitin conjugating enzyme E2 S S homeolog Protein, ubiquitin conjugating enzyme E2 S L homeolog Protein, UBE2S Protein, Ube2s Protein, ube2s Protein, ATEG_00324 Protein, Smp_180170 Protein, MCYG_01532 Protein, ube2s.S Protein, ube2s.L Protein

Background:

Background: Ubiquitin-Conjugating Enzyme E2 S (UBE2S) is a member of the Ubiquitin-Conjugating Enzyme family. UBE2S interacts with CDC20, FZR1/CDH1 and VHL. UBE2S can form a thiol ester linkage with Ubiquitin in an Ubiquitin Activating Enzyme-Dependent manner, a characteristic property of Ubiquitin Carrier Proteins. UBE2S acts as an essential factor of the Anaphase Promoting Complex/Cyclosome, a cell cycle-regulated Ubiquitin ligase that controls progression through mitosis. UBE2S is also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A.

Synonym: Ubiquitin-Conjugating Enzyme E2 S, E2-EPF, Ubiquitin Carrier Protein S, Ubiquitin-Conjugating Enzyme E2-24 kDa, Ubiquitin-Conjugating Enzyme E2-EPF5, Ubiquitin-Protein Ligase S, UBE2S, E2EPF

Molecular Weight: 50.1 kDa

UniProt: Q16763

Pathways: Ubiquitin Proteasome Pathway