UCHL1 Protein (AA 1-223) (His tag)
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- Target See all UCHL1 Proteins
- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-223
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UCHL1 protein is labelled with His tag.
- Purpose
- Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1/UCH-L1 (C-6His)
- Sequence
- MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAALEHHHHH H
- Characteristics
- Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1/UCH-L1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ala223) of Human UCH-L1.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product UCHL1 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 250 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 7.5.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
- Alternative Name
- uch-l1 (UCHL1 Products)
- Synonyms
- PARK5 Protein, PGP 9.5 Protein, PGP9.5 Protein, PGP95 Protein, Uch-L1 Protein, UCH-L1 Protein, UCHL1 Protein, cb358 Protein, wu:fc55h08 Protein, park5 Protein, pgp9.5 Protein, uch-l1 Protein, MGC132191 Protein, uchl1 Protein, AW822034 Protein, C88048 Protein, R75593 Protein, UCHL-1 Protein, gad Protein, ubiquitin C-terminal hydrolase L1 Protein, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) Protein, ubiquitin C-terminal hydrolase L1 L homeolog Protein, ubiquitin carboxyl-terminal esterase L1 Protein, ubiquitin carboxy-terminal hydrolase L1 Protein, UCHL1 Protein, uchl1 Protein, uchl1.L Protein, LOC100304750 Protein, Uchl1 Protein
- Background
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Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.
Alternative Names: Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1, UCHL1 - Molecular Weight
- 25.89 kDa
- UniProt
- P09936
- Pathways
- Feeding Behaviour
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