MRRF Protein (His tag)
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- Target See all MRRF Proteins
- MRRF (Mitochondrial Ribosome Recycling Factor (MRRF))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MRRF protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product MRRF Protein
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- Comment
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Synonyms: Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 30% glycerol, 2 mM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MRRF (Mitochondrial Ribosome Recycling Factor (MRRF))
- Alternative Name
- MRRF (MRRF Products)
- Synonyms
- MRRF Protein, zgc:91914 Protein, mrrf Protein, MGC147174 Protein, MRFF Protein, MTRRF Protein, RRF Protein, 2400002D02Rik Protein, mitochondrial ribosome recycling factor Protein, ribosome-recycling factor, mitochondrial Protein, Mitochondrial ribosome recycling factor Protein, MRRF Protein, mrrf Protein, LOC5571701 Protein, PAS_chr4_0253 Protein, Mrrf Protein
- Background
- MRRF binds to the large ribosomal subunit in the cleft that contains the peptidyl transferase center. This protein is responsible for the release of ribosome from messenger RNA at the termination of protein biosynthesis. Also, it may increase the efficiency of translation by recycling ribosome from one round of translation to another. Recombinant human MRRF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 25.1 kDa (228aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_620132
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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