TGFB2 Protein (His tag)
(4 references)-
- Target See all TGFB2 Proteins
- TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Tobacco (Nicotiana benthamiana)
- Purification tag / Conjugate
- This TGFB2 protein is labelled with His tag.
- Application
- Western Blotting (WB), Immunogen (Imm), ELISA, Standard (STD)
- Sequence
- HHHHHHALDA AYCFRNVQDN CCLRPLYIDF KRDLGWKWIH EPKGYNANFC AGACPYLWSS DTQHSRVLSL YNTINPEASA SPCCVSQDLE PLTILYYIGK TPKIEQLSNM IVKSCKCS
- Specificity
- Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta2.
- Characteristics
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Recombinant human TGF beta 2 contains a 6-His tag at the N-terminal end, is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal free product.
Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta2.
Molecular Formula: C602H909N167O171S10
Isoelectric Point: 7,72
Extinction Coefficient: E 0.1 % (1g/L) = 2.02 (A 280 nm)
This product contains no animal-derived components or impurities. It is produced by transient expression of TGF-beta3 in non-transgenic plants. - Purification
- Recombinant human TGF-beta3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).
- Purity
- > 97 % by SDS-PAGE gel
- Endotoxin Level
- < 0.04 EU/µg protein (LAL method)
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Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein
TGFB2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein (AVI tag,Biotin)TGFB2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 2 (TGFB2) (AA 1-414) protein (GST tag)TGFB2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Transforming Growth Factor, beta 2 (TGFB2) (Active) proteinTGFB2 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein (His tag)Crystallography grade TGFB2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, beta 2 (TGFB2) (AA 21-302) protein (His tag)Crystallography grade TGFB2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein (His tag)Crystallography grade TGFB2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, beta 2 (TGFB2) (AA 21-302) protein (His tag)Crystallography grade TGFB2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Comment
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Human recombinant protein expressed in Nicotiana benthamiana.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng /µL. Due to the protein nature, dimmers and multimers may be observed. Upon reconstitution, It can be stored in working aliquots at -20 °C for future use.
- Concentration
- 50 ng/µL
- Buffer
- Tris HCl 0.05 M buffer at pH 7.4.
- Handling Advice
- Reconstituted protein should be stored in working aliquots at -20 °C and it is recommended to add a carrier protein (0.1 % HSA or BSA). Repeated freezing and thawing is not recommended.
- Storage
- 4 °C
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: "An improved recombinant mammalian cell expression system for human transforming growth factor-beta2 and -beta3 preparations." in: Protein expression and purification, Vol. 50, Issue 1, pp. 9-17, (2006) (PubMed).
: "The transforming growth factor-beta family." in: Annual review of cell biology, Vol. 6, pp. 597-641, (1991) (PubMed).
: "Transforming growth factor-beta. A family of growth regulatory peptides." in: Annals of the New York Academy of Sciences, Vol. 593, pp. 208-17, (1990) (PubMed).
: "Identification of another member of the transforming growth factor type beta gene family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 13, pp. 4715-9, (1988) (PubMed).
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: "An improved recombinant mammalian cell expression system for human transforming growth factor-beta2 and -beta3 preparations." in: Protein expression and purification, Vol. 50, Issue 1, pp. 9-17, (2006) (PubMed).
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- Target
- TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
- Alternative Name
- TGF beta 2 (TGFB2 Products)
- Background
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Synonyms: Transforming growth factor beta-2, TGF-beta-2
Recombinant human TGF-beta2 is a 27.08 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulphide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2 and TGF-beta3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing. - Molecular Weight
- 27.08 kDa
- Gene ID
- 7042, 190220
- UniProt
- P61812
- Pathways
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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