CRYAA Protein (AA 1-173) (His tag)
-
- Target See all CRYAA Proteins
- CRYAA (Crystallin, alpha A (CRYAA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-173
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CRYAA protein is labelled with His tag.
- Purpose
- Recombinant Human α-Crystallin A Chain/CRYAA (C-6His)
- Sequence
- MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRL PSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSSLEHHHHH H
- Characteristics
- Recombinant Human Alpha-Crystallin A Chain/CRYAA is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ser173) of Human CRYAA fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CRYAA Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, 2 mM EDTA, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
-
- Target
- CRYAA (Crystallin, alpha A (CRYAA))
- Alternative Name
- CRYAA (CRYAA Products)
- Synonyms
- CRYA1 Protein, CTRCT9 Protein, HSPB4 Protein, AI323437 Protein, Acry-1 Protein, Crya-1 Protein, Crya1 Protein, DAcry-1 Protein, lop18 Protein, alpha-a-crystallin Protein, cryaa-A Protein, CRYAA Protein, wu:fc06h04 Protein, zgc:92036 Protein, HspB4 Protein, crystallin alpha A Protein, crystallin, alpha A Protein, alphaA-crystallin Protein, alpha-crystallin A chain-like Protein, crystallin alpha A S homeolog Protein, alpha-crystallin A chain Protein, CRYAA Protein, Cryaa Protein, CpipJ_CPIJ005646 Protein, cryaa Protein, LOC100384978 Protein, cryaa.S Protein, LOC458589 Protein, LOC102724652 Protein
- Sub Type
- Fusionprotein
- Background
-
Alpha-Crystallin A Chain (CRYAA) belongs to the small heat shock protein (HSP20) family and can be induced by heat shock. The expression of CRYAA is preferentially restricted to the lens cell. CRYAA may contribute to the transparency and refractive index of the lens. CRYAA has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. Two additional functions of CRYAA are an autokinase activity and participation in the intracellular architecture.
Alternative Names: Alpha-Crystallin A Chain, Heat Shock Protein Beta-4, HspB4, Alpha-Crystallin A Chain, Short Form, CRYAA, CRYA1, HSPB4 - Molecular Weight
- 20.9 kDa
- UniProt
- P02489
- Pathways
- M Phase
-