PAD4 Protein (AA 1-663) (His tag)

Catalog No. ABIN1691945

Product Details

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Protein Type: Recombinant

Protein Characteristics: AA 1-663

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PAD4 protein is labelled with His tag.

Purpose: Recombinant Human Protein-Arginine Deiminase Type-4/PADI4 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAQGTLIRVT PEQPTHAVCV LGTLTQLDIC SSAPEDCTSF SINASPGVVV DIAHSPPAKK KSTGSSTWPL DPGVEVTLTM KAASGSTGDQ KVQISYYGPK TPPVKALLYL TAVEISLCAD ITRTGKVKPT RAVKDQRTWT WGPCGQGAIL LVNCDRDNLE SSAMDCEDDE VLDSEDLQDM SLMTLSTKTP KDFFTNHTLV LHVARSEMDK VRVFQATRGK LSSKCSVVLG PKWPSHYLMV PGGKHNMDFY VEALAFPDTD FPGLITLTIS LLDTSNLELP EAVVFQDSVV FRVAPWIMTP NTQPPQEVYA CSIFENEDFL KSVTTLAMKA KCKLTICPEE ENMDDQWMQD EMEIGYIQAP HKTLPVVFDS PRNRGLKEFP IKRVMGPDFG YVTRGPQTGG ISGLDSFGNL EVSPPVTVRG KEYPLGRILF GDSCYPSNDS RQMHQALQDF LSAQQVQAPV KLYSDWLSVG HVDEFLSFVP APDRKGFRLL LASPRSCYKL FQEQQNEGHG EALLFEGIKK KKQQKIKNIL SNKTLREHNS FVERCIDWNR ELLKRELGLA ESDIIDIPQL FKLKEFSKAE AFFPNMVNML VLGKHLGIPK PFGPVINGRC CLEEKVCSLL EPLGLQCTFI NDFFTYHIRH GEVHCGTNVR RKPFSFKWWN MVP

Characteristics:

Recombinant Human Protein-Arginine Deiminase Type-4/PADI4 (N-6His)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 500 mM NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Alternative Name: Protein-Arginine Deiminase Type-4/PADI4 (PADI4 Products)

Synonyms: PAD Protein, PAD4 Protein, PADI5 Protein, PDI4 Protein, PDI5 Protein, Pad4 Protein, Pdi4 Protein, peptidyl arginine deiminase 4 Protein, peptidyl arginine deiminase, type IV Protein, PADI4 Protein, Padi4 Protein

Background: Recombinant Human Protein-Arginine Deiminase Type-4/PADI4 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Pro663) of Human PADI4 fused with a 6His tag at the N-terminus.
Protein-Arginine Deiminase Type-4 (PADI4) belongs to the Protein Arginine Deiminase family. PADI4 is expressed in eosinophils and neutrophils, but it is not expressed in peripheral monocytes or lymphocytes. PADI4 catalyzes the citrullination/deimination of arginine residues of proteins. In addition, PADI4 may play an important role in tumourigenesis. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA), which is a systemic inflammatory disease with autoimmune features and a complex genetic component.

Molecular Weight: 76.2 kDa

UniProt: Q9UM07