VCP Protein (AA 2-589) (His tag)
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- Target See all VCP Proteins
- VCP (Valosin Containing Protein (VCP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-589
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This VCP protein is labelled with His tag.
- Purpose
- Recombinant Human Valosin-Containing Protein/VCP (C-6His)
- Sequence
- ASGADSKGDD LSTAILKQKN RPNRLIVDEA INEDNSVVSL SQPKMDELQL FRGDTVLLKG KKRREAVCIV LSDDTCSDEK IRMNRVVRNN LRVRLGDVIS IQPCPDVKYG KRIHVLPIDD TVEGITGNLF EVYLKPYFLE AYRPIRKGDI FLVRGGMRAV EFKVVETHPS PYCIVAPDTV IHCEGEPIKR EDEEESLNEV GYDDIGGCRK QLAQIKEMVE LPLRHPALFK AIGVKPPRGI LLYGPPGTGK TLIARAVANE TGAFFFLING PEIMSKLAGE SESNLRKAFE EAEKNAPAII FIDELDAIAP KREKTHGEVE RRIVSQLLTL MDGLKQRAHV IVMAATNRPN SIDPALRRFG RFDREVDIGI PDATGRLEIL QIHTKNMKLA DDVDLEQVAN ETHGHVGADL AALCSEAALQ AIRKKMDLID LEDETIDAEV MNSLAVTMDD FRWALSQSNP SALRETVVEV PQVTWEDIGG LEDVKRELQE LVQYPVEHPD KFLKFGMTPS KGVLFYGPPG CGKTLLAKAI ANECQANFIS IKGPELLTMW FGESEANVRE IFDKARQAAP CVLFFDELDS IAKARGGNVE HHHHHH
- Characteristics
- Recombinant Human Valosin-Containing Protein/VCP is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Asn589) of Human VCP fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product VCP Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- VCP (Valosin Containing Protein (VCP))
- Alternative Name
- VCP (VCP Products)
- Synonyms
- ALS14 Protein, IBMPFD Protein, TERA Protein, p97 Protein, CDC48 Protein, wu:fd16d05 Protein, wu:fj63d11 Protein, p93 Protein, 3110001E05 Protein, p97/VCP Protein, 22.26 Protein, 7.12 Protein, Apple Protein, BcDNA.GM02885 Protein, BcDNA:GM02885 Protein, CG2331 Protein, DmTER94 Protein, Dmel\CG2331 Protein, I Protein, Ter94 Protein, Tera Protein, VCP Protein, VCP ATPase Protein, VCP/p97 Protein, Vcp Protein, anon-WO2004063362.65 Protein, anon-WO2004063362.67 Protein, cdc48 Protein, dTERA Protein, dVCP Protein, dmTERA Protein, dvcp Protein, l(2)03775 Protein, l(2)46CFf Protein, l(2)46CFs Protein, l(2)46Ch Protein, pTER94 Protein, ter94 Protein, vcp Protein, FANCG Protein, valosin containing protein Protein, valosin containing protein S homeolog Protein, valosin-containing protein Protein, CG2331 gene product from transcript CG2331-RA Protein, zgc:136908 Protein, transitional endoplasmic reticulum ATPase Protein, VCP Protein, vcp.S Protein, vcp Protein, Vcp Protein, Bm1_56585 Protein, LOC100136246 Protein, TER94 Protein, zgc:136908 Protein, LOC101109680 Protein
- Sub Type
- Fusionprotein
- Background
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Valosin-Containing Protein (VCP) is a nuclear protein that belongs to the AAA ATPase family. VCP is a putative ATP-binding protein involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. It is necessary for the fragmentation of Golgi stacks during mitosis and their reassembly after mitosis. VCP has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. VCP participates in the formation of the transitional endoplasmic reticulum (tER) and regulates E3 ubiquitin-protein ligase activity of RNF19A.
Alternative Names: Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit, Valosin-Containing Protein, VCP - Molecular Weight
- 66.4 kDa
- UniProt
- P55072
- Pathways
- ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
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