Chemokine (C-X-C Motif) Ligand 10 (CXCL10) (AA 22-98) (Active) Protein

Details for Product No. ABIN2666633, Supplier: Log in to see
Protein Name
  • CXCL10
  • C7
  • IFI10
  • INP10
  • IP-10
  • SCYB10
  • crg-2
  • gIP-10
  • mob-1
  • CRG-2
  • IP10
  • Ifi10
  • Scyb10
  • chemokine (C-X-C motif) ligand 10
  • C-X-C motif chemokine 10
  • CXCL10
  • cxl10
  • Cxcl10
Protein Characteristics
AA 22-98
26
2
2
1
1
1
1
Origin
Mouse (Murine)
46
19
8
3
3
1
1
1
Source
Escherichia coli (E. coli)
61
7
2
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Multiplex Assay (MA)
Options
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Purity > 98 % , as determined by Coomassie stained SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Background CXCL10 is an ELR-negative chemokine structurally and functionally related to CXCL9 and CXCL11. CXCL10, CXCL9, and CXCL11 are produced and secreted by monocytes, macrophages, fibroblasts, and epithelial cells upon stimulation with proinflammatory cytokines, especially IFNγ. CXCL10 chemoattracts CD4, CD8, and NK and NKT cells through the binding to its receptor CXCR3, which is shared with CXCL9 and CXCL11. In addition, CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. Also, CXCL10 has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the anti-proliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through GAG interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. CXCL10 expression is strongly upregulated in many inflammatory diseases, including arthritis, type I diabetes, experimental autoimmune encepahlomyelitis, atherosclerosis, allograft rejection, and others.
Molecular Weight The 78 amino acid recombinant protein has a predicted molecular mass of approximately 8.8 kDa. The DTT-reduced and non-reduced protein migrate at approximately 10 kDa by SDS-PAGE. The N-terminal amino acid is Met.
Research Area Chemokines
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Biological activity: Bioactivity was measured by its property to chemoattract PHA, IL-2 activated T cells in a dose dependent manner.

Restrictions For Research Use only
Format Liquid
Reconstitution For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
Buffer 0.22 μm filtered protein solution is in PBS.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage -20 °C
Storage Comment Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
Supplier Images
ELISA image for Chemokine (C-X-C Motif) Ligand 10 (CXCL10) (AA 22-98) (Active) protein (ABIN2666633) Chemokine (C-X-C Motif) Ligand 10 (CXCL10) (AA 22-98) (Active) protein
Background publications Hoerning, Koss, Datta, Boneschansker, Jones, Wong, Irimia, Calzadilla, Benitez, Hoyer, Harmon, Briscoe: "Subsets of human CD4(+) regulatory T cells express the peripheral homing receptor CXCR3." in: European journal of immunology, Vol. 41, Issue 8, pp. 2291-302, 2011 (PubMed).

Campanella, Colvin, Luster: "CXCL10 can inhibit endothelial cell proliferation independently of CXCR3." in: PLoS ONE, Vol. 5, Issue 9, pp. e12700, 2010 (PubMed).

Crawford, Zhu, Green, Burdick, Sanz, Alem, OBrien, Mehrad, Strieter, Hughes: "Antimicrobial effects of interferon-inducible CXC chemokines against Bacillus anthracis spores and bacilli." in: Infection and immunity, Vol. 77, Issue 4, pp. 1664-78, 2009 (PubMed).

Loos, Mortier, Gouwy, Ronsse, Put, Lenaerts, Van Damme, Proost: "Citrullination of CXCL10 and CXCL11 by peptidylarginine deiminase: a naturally occurring posttranslational modification of chemokines and new dimension of immunoregulation." in: Blood, Vol. 112, Issue 7, pp. 2648-56, 2008 (PubMed).

Vanguri, Farber: "Identification of CRG-2. An interferon-inducible mRNA predicted to encode a murine monokine." in: The Journal of biological chemistry, Vol. 265, Issue 25, pp. 15049-57, 1990 (PubMed).

Luster, Unkeless, Ravetch: "Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins." in: Nature, Vol. 315, Issue 6021, pp. 672-6, 1985 (PubMed).

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