AUH Protein (Myc-DYKDDDDK Tag)
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- Target See all AUH Proteins
- AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This AUH protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human AUH protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product AUH Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))
- Alternative Name
- Auh (AUH Products)
- Synonyms
- C77140 Protein, W91705 Protein, wu:fb81b10 Protein, zgc:101057 Protein, AU RNA binding methylglutaconyl-CoA hydratase Protein, AU RNA binding protein/enoyl-coenzyme A hydratase Protein, AU RNA binding protein/enoyl-CoA hydratase Protein, AU RNA binding protein/enoyl-CoA hydratase S homeolog Protein, AUH Protein, Auh Protein, auh Protein, auh.S Protein
- Background
- This gene encodes bifunctional mitochondrial protein that has both RNA-binding and hydratase activities. The encoded protein is a methylglutaconyl-CoA hydratase that catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methyl-glutaryl-CoA, a critical step in the leucine degradation pathway. This protein also binds AU-rich elements (AREs) found in the 3' UTRs of rapidly decaying mRNAs including c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. This protein is localizes to the mitochondrial matrix and the inner mitochondrial membrane and may be involved in mitochondrial protein synthesis. Mutations in this gene are the cause of 3-methylglutaconic aciduria, type I. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 29.1 kDa
- NCBI Accession
- NP_001689
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