beta Defensin 1 Protein

Catalog No. ABIN2719355

Product Details

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Defensin beta 1 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 100.0-1000.0 ng/ml.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Alternative Name: Defensin beta 1 (DEFB1 Products)

Synonyms: BD1 Protein, DEFB-1 Protein, DEFB101 Protein, HBD1 Protein, AW260221 Protein, BD-1 Protein, DEFB1-like Protein, RHBD-1 Protein, CBD1 Protein, PBD-1 Protein, DEFB1 Protein, BNBD-1 Protein, defensin beta 1 Protein, avian beta-defensin 1 Protein, defensin, beta 1 Protein, beta-defensin 1-like Protein, beta-defensin 1 Protein, DEFB1 Protein, Defb1 Protein, AvBD1 Protein, LOC100861170 Protein

Background: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis.

Molecular Weight: 5 kDa

NCBI Accession: NP_005209