HSP70 1A Protein (His tag)
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- Target See all HSP70 1A (HSPA1A) Proteins
- HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This HSP70 1A protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Heat shock 70 kDa protein 1A (HSPA1A), full length, with C-terminal polyhistidine tag, expressed in sf9 cells. (full length, C-term polyhistidine tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product HSPA1A Protein
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Heat Shock 70kDa Protein 1A (HSPA1A) (AA 291-641) protein (His-IF2DI Tag)
HSPA1A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Heat Shock 70kDa Protein 1A (HSPA1A) (Active) protein (His tag)HSPA1A Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE. Active
Heat Shock 70kDa Protein 1A (HSPA1A) (Active) protein (Myc-DYKDDDDK Tag)HSPA1A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 20 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
- Abstract
- HSPA1A Products
- Synonyms
- hsp70-4 Protein, hspa1a Protein, Hsp70-3 Protein, Hsp70.3 Protein, Hsp72 Protein, hsp68 Protein, hsp70A1 Protein, HSP72 Protein, Hsp70-1 Protein, Hspa1 Protein, Hspa1b Protein, hsp70-1 Protein, hsp70-1a Protein, hsp70i Protein, hsp72 Protein, hspa1 Protein, hspa1b Protein, HSP70-1 Protein, HSP70-1A Protein, HSP70I Protein, HSPA1 Protein, HSPA1A Protein, HSPA1B Protein, HSP70-2 Protein, HSPA2 Protein, HSP70 Protein, hspA1A Protein, heat shock cognate 70-kd protein, tandem duplicate 3 Protein, heat shock 70 kDa protein 1 Protein, heat shock 70 kDa protein II Protein, heat shock protein 1A Protein, heat shock 70kD protein 1A Protein, heat shock protein family A (Hsp70) member 1A S homeolog Protein, heat shock protein family A (Hsp70) member 1A Protein, heat shock protein 70.2 Protein, heat shock 70kDa protein 1A Protein, heat shock 70 kDa protein 1B Protein, heat shock protein 70.1 Protein, hsp70.3 Protein, LOC100023597 Protein, LOC100554002 Protein, LOC100608888 Protein, Hspa1a Protein, hspa1a.S Protein, HSPA1A Protein, HSP70.2 Protein, LOC100354037 Protein, HSP70.1 Protein
- Background
- This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
- Molecular Weight
- 70 kDa
- NCBI Accession
- NP_005336
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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