HNF4A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722902

Product Details

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HNF4A protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human HNF4 alpha / TCF14 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: HNF4A Activity Verified in a DNA-binding Assay: HNF4A (, transcript variant 2) activity was measured in a colorimetric DNA-binding assay. Purified HNF4A protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the HNF4A consensus DNA-binding sequence (see below). Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Ning, Koh, Pan, Jeong: "Hepatocyte nuclear factor (HNF) 4α transactivation of cytochrome P450 (Cyp) 2d40 promoter is enhanced during pregnancy in mice." in: Biochemical pharmacology, Vol. 94, Issue 1, pp. 46-52, (2015) (PubMed).

Target Details

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: Hnf4 Alpha,tcf14 (HNF4A Products)

Synonyms: HNF4A Protein, HNF4alpha Protein, CG9310 Protein, DmHNF4 Protein, Dmel\\CG9310 Protein, HNF-4 Protein, HNF-4(D) Protein, HNF4 Protein, Hnf-4 Protein, Hnf-4h Protein, NR2A4 Protein, dHNF-4 Protein, dHNF4 Protein, Hnf4 Protein, Hnf4alpha Protein, MODY1 Protein, Nr2a1 Protein, Tcf14 Protein, HNF4a7 Protein, HNF4a8 Protein, HNF4a9 Protein, MODY Protein, NR2A1 Protein, NR2A21 Protein, TCF Protein, TCF14 Protein, HNF-4alpha Protein, fb58h09 Protein, id:ibd1279 Protein, wu:fb58h09 Protein, hnf4 Protein, nr2a1 Protein, hepatocyte nuclear factor 4 alpha Protein, Hepatocyte nuclear factor 4 Protein, hepatic nuclear factor 4, alpha Protein, hepatocyte nuclear factor 4, alpha Protein, hepatocyte nuclear factor 4 alpha S homeolog Protein, HNF4A Protein, hnf4a Protein, Hnf4 Protein, Hnf4a Protein, hnf4a.S Protein

Background: Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 52.6 kDa

NCBI Accession: NP_000448

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process